Development of a combined RT-PCR: NGS assay for microRNA biomarkers

William John Andrews, Eoin Brown, Margaret Dellett, David Arthur Simpson

Research output: Contribution to conferencePoster

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Abstract

The ability of miRNAs to act as diagnostic biomarkers could be expanded by availability of improved methodologies to detect and analyse these molecules. We have therefore developed an assay with the ability to selectively analyse pools of miRNAs, using the specificity of PCR to select targets and the power of NGS to reveal isomiRs of the chosen targets in a total assay time of two days.
Original languageEnglish
Number of pages1
Publication statusPublished - 16 Mar 2015
EventCambridge Healthtech Institute's Extracellular Biomarkers Summit - Hyatt Regency Cambridge, Boston, United States
Duration: 16 Mar 201518 Mar 2015

Conference

ConferenceCambridge Healthtech Institute's Extracellular Biomarkers Summit
CountryUnited States
CityBoston
Period16/03/201518/03/2015

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