Development of a combined RT-PCR: NGS assay for microRNA biomarkers

William John Andrews; Eoin Brown; Margaret Dellett; David Arthur Simpson

Development of a combined RT-PCR: NGS assay for microRNA biomarkers

William John Andrews, Eoin Brown, Margaret Dellett, David Arthur Simpson

School of Medicine, Dentistry and Biomedical Sciences

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Abstract

The ability of miRNAs to act as diagnostic biomarkers could be expanded by availability of improved methodologies to detect and analyse these molecules. We have therefore developed an assay with the ability to selectively analyse pools of miRNAs, using the specificity of PCR to select targets and the power of NGS to reveal isomiRs of the chosen targets in a total assay time of two days.

Original language	English
Number of pages	1
Publication status	Published - 16 Mar 2015
Event	Cambridge Healthtech Institute's Extracellular Biomarkers Summit - Hyatt Regency Cambridge, Boston, United States Duration: 16 Mar 2015 → 18 Mar 2015

Conference

Conference	Cambridge Healthtech Institute's Extracellular Biomarkers Summit
Country/Territory	United States
City	Boston
Period	16/03/2015 → 18/03/2015

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Development of a combined RT-PCR: NGS assay for microRNA biomarkers
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http://www.extracellularbiomarkers.com/

Cite this

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title = "Development of a combined RT-PCR: NGS assay for microRNA biomarkers",

abstract = "The ability of miRNAs to act as diagnostic biomarkers could be expanded by availability of improved methodologies to detect and analyse these molecules. We have therefore developed an assay with the ability to selectively analyse pools of miRNAs, using the specificity of PCR to select targets and the power of NGS to reveal isomiRs of the chosen targets in a total assay time of two days.",

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AU - Andrews, William John

AU - Brown, Eoin

AU - Dellett, Margaret

AU - Simpson, David Arthur

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Y1 - 2015/3/16

N2 - The ability of miRNAs to act as diagnostic biomarkers could be expanded by availability of improved methodologies to detect and analyse these molecules. We have therefore developed an assay with the ability to selectively analyse pools of miRNAs, using the specificity of PCR to select targets and the power of NGS to reveal isomiRs of the chosen targets in a total assay time of two days.

AB - The ability of miRNAs to act as diagnostic biomarkers could be expanded by availability of improved methodologies to detect and analyse these molecules. We have therefore developed an assay with the ability to selectively analyse pools of miRNAs, using the specificity of PCR to select targets and the power of NGS to reveal isomiRs of the chosen targets in a total assay time of two days.

M3 - Poster

T2 - Cambridge Healthtech Institute's Extracellular Biomarkers Summit

Y2 - 16 March 2015 through 18 March 2015

ER -

Development of a combined RT-PCR: NGS assay for microRNA biomarkers

Abstract

Conference

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