Development of a mAb library for the diagnosis of melioidosis.

Michael Dillon, Dana E Reed, Raymond L Houghton, S. Raychaudhuri, J. Chen, Paul J Brett, Mary N Burtnick, David P AuCoin

Research output: Contribution to conferencePosterpeer-review

Abstract

Burkholderia pseudomallei, the causative agent of melioidosis, produces a high molecular weight capsular polysaccharide (CPS) comprised of an unbranched homopolymer of 1,3-linked 2-O-acetyl-6-deoxy-β-D-mannoheptopyranose residues. Our laboratory has previously produced a CPS specific IgG3 monoclonal antibody (mAb), 3C5, and determined that it is able to provide passive protection in a murine model of pulmonary melioidosis. In addition, the mAb was used to detect shed CPS in melioidosis patient samples and was successfully incorporated into a prototype Active Melioidosis DetectTM Lateral Flow Immunoassay (AMD LFI) through a collaboration with our laboratory and InBios International, Inc. While a successful prototype was produced using mAb 3C5, background issues attributed to IgG3 self association lead us to pursue making additional antibodies of alternate isotypes. We have recently isolated several murine IgG1 mAbs targeting CPS by immunizing BALB/c mice with a CPS glycoconjugate. The new library of mAbs show a range in CPS binding affinities, with one mAb in particular, 4C4, having a considerably higher affinity for CPS compared to our previous IgG3 mAb. An updated LFI incorporating a new CPS-specific IgG1 displays high CPS sensitivity with greatly reduced background signal, highlighting its potential as a sensitive and specific rapid diagnostic for melioidosis.
Original languageEnglish
Publication statusPublished - 17 Nov 2013
Externally publishedYes
Event9th Annual Pacific Southwest Regional Centre of Excellence Meeting - Shorebreak Hotel, Huntington Beach , United States
Duration: 17 Nov 201319 Nov 2019

Conference

Conference9th Annual Pacific Southwest Regional Centre of Excellence Meeting
Abbreviated titlePSWRCE 9
CountryUnited States
CityHuntington Beach
Period17/11/201319/11/2019

Keywords

  • Antibody
  • Burkholderia pseudomallei
  • lateral flow assay
  • Diagnostic
  • Melioidosis
  • Lateral Flow Immunoassay
  • Immunoglobulin Class Switching
  • IMMUNOGLOBULIN
  • infectious disease
  • emerging infectious disease
  • tropical disease
  • neglected disease
  • immunoassay
  • bioterrorism

Fingerprint Dive into the research topics of 'Development of a mAb library for the diagnosis of melioidosis.'. Together they form a unique fingerprint.

  • Travel Grant

    Dillon, M. (Recipient), 18 Sep 2013

    Prize: Prize (including medals and awards)

Cite this