Development of a multi-residue high-throughput UHPLC-MS/MS method for routine monitoring of SARM compounds in equine and bovine blood

Emiliano Ventura, Anna Gadaj, Tom Buckley, Mark H Mooney

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
53 Downloads (Pure)

Abstract

Selective androgen receptor modulators (SARMs) are a group of anabolic enhancer drugs posing threats to the integrity of animal sports and the safety of animal-derived foods. The current research describes for the first time the development of a semi-quantitative assay for the monitoring of SARM family compounds in blood and establishes the relative stability of these analytes under various storage conditions prior to analysis. The presented screening method validation was performed in line with current EU legislation for the inspection of livestock and produce of animal origin, with detection capability (CCβ) values determined at 0.5 ng/mL (Ly2452473), 1 ng/mL (AC-262536 and PF-06260414), 2 ng/mL (bicalutamide, GLPG0492, LGD-2226, ostarine, S-1, S-6, and S-23), and 5 ng/mL (andarine, BMS-564929, LGD-4033, RAD140, and S-9), respectively. The applicability of the developed assay was demonstrated through the analysis of blood samples from racehorses and cattle. The developed method presents a high-throughput cost-effective tool for the routine screening for a range of SARM compounds in sport and livestock animals.

Original languageEnglish
JournalDrug Testing and Analysis
Early online date10 Jun 2020
DOIs
Publication statusEarly online date - 10 Jun 2020

Bibliographical note

© 2020 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.

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