Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

D. Romanazzo, F. Ricci, G. Volpe, Christopher Elliott, S. Vesco, K. Kroeger, D. Moscone, J. Stroka, H. Van Egmond, M. Vehniainen, G. Palleschi

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

A reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab fragment is presented. The analytical system employed, Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers.
Original languageEnglish
Pages (from-to)2615-2621
Number of pages7
JournalBiosensors and Bioelectronics
Volume25
Issue number12
DOIs
Publication statusPublished - Aug 2010

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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