Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

D. Romanazzo; F. Ricci; G. Volpe; Christopher Elliott; S. Vesco; K. Kroeger; D. Moscone; J. Stroka; H. Van Egmond; M. Vehniainen; G. Palleschi

doi:10.1016/j.bios.2010.04.029

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

D. Romanazzo, F. Ricci, G. Volpe, Christopher Elliott, S. Vesco, K. Kroeger, D. Moscone, J. Stroka, H. Van Egmond, M. Vehniainen, G. Palleschi

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

A reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab fragment is presented. The analytical system employed, Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers.

Original language	English
Pages (from-to)	2615-2621
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	25
Issue number	12
DOIs	https://doi.org/10.1016/j.bios.2010.04.029
Publication status	Published - Aug 2010

ASJC Scopus subject areas

Biophysics
Biomedical Engineering
Biotechnology
Electrochemistry

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10.1016/j.bios.2010.04.029

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Romanazzo, D., Ricci, F., Volpe, G., Elliott, C., Vesco, S., Kroeger, K., Moscone, D., Stroka, J., Van Egmond, H., Vehniainen, M., & Palleschi, G. (2010). Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples. Biosensors and Bioelectronics, 25(12), 2615-2621. https://doi.org/10.1016/j.bios.2010.04.029

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abstract = "A reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab fragment is presented. The analytical system employed, Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers.",

author = "D. Romanazzo and F. Ricci and G. Volpe and Christopher Elliott and S. Vesco and K. Kroeger and D. Moscone and J. Stroka and {Van Egmond}, H. and M. Vehniainen and G. Palleschi",

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language = "English",

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Romanazzo, D, Ricci, F, Volpe, G, Elliott, C, Vesco, S, Kroeger, K, Moscone, D, Stroka, J, Van Egmond, H, Vehniainen, M & Palleschi, G 2010, 'Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples', Biosensors and Bioelectronics, vol. 25, no. 12, pp. 2615-2621. https://doi.org/10.1016/j.bios.2010.04.029

TY - JOUR

T1 - Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

AU - Romanazzo, D.

AU - Ricci, F.

AU - Volpe, G.

AU - Elliott, Christopher

AU - Vesco, S.

AU - Kroeger, K.

AU - Moscone, D.

AU - Stroka, J.

AU - Van Egmond, H.

AU - Vehniainen, M.

AU - Palleschi, G.

PY - 2010/8

Y1 - 2010/8

N2 - A reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab fragment is presented. The analytical system employed, Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers.

AB - A reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab fragment is presented. The analytical system employed, Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers.

U2 - 10.1016/j.bios.2010.04.029

DO - 10.1016/j.bios.2010.04.029

M3 - Article

SN - 0956-5663

VL - 25

SP - 2615

EP - 2621

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 12

ER -

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

Abstract

ASJC Scopus subject areas

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