Development of amphiphilic block copolymers as silica optical fiber overlayers for BSA protein detection

Afroditi Petropoulou, Thomas J. Gibson, Efrosyni Themistou, Stergios Pispas, Christos Riziotis

Research output: Contribution to journalArticle

5 Citations (Scopus)
154 Downloads (Pure)

Abstract

Novel amphiphilic block copolymers having both hydrophobic poly(methyl methacrylate) (PMMA) and hydrophilic poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) blocks have been designed and synthesized for efficient protein detection in photonic-based sensing. Both the cationic PMMA117-b-PDMAEMA16 and the cationic vinyl-sulfone functionalized PMMA117-b-P(DMAEMA17-VSTEMA2) block copolymers were synthesized from a water insoluble hydrophobic PMMA block, which facilitated the formation of stable overlayers on the silica optical fibers surface. The well-defined structure of the co-polymers was confirmed by gel permeation chromatography (GPC). The presence of the cationic PDMAEMA block and the vinyl-sulfone double bonds led to reversible electrostatic binding of negatively charged proteins like bovine serum albumin (BSA) and non-reversible chemical binding by thiol-ene reactions with cysteine in proteins, respectively. The sensing properties of these materials were assessed and confirmed by ATR-FTIR analysis and by the characterization of fabricated sensing heads on silica optical fibers functionalized with suitably deposited overlayers. The sensing assessment revealed the requirements for deposited overlayer characteristics towards proteins' detection sensitivity and selectivity enhancement.
Original languageEnglish
Pages (from-to)421-428
JournalMaterials Chemistry and Physics
Volume216
Early online date11 Jun 2018
DOIs
Publication statusPublished - 01 Sep 2018

Keywords

  • Biosensing
  • Block copolymers
  • Optical fibers
  • Photonic sensors
  • Polymers
  • Proteins

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Materials Science(all)

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