Development of an optimized broth enrichment culture medium for the isolation of Clostridium difficile

Mairéad C Connor, John W McGrath, Geoff McMullan, Nikki Marks, Derek J Fairley

Research output: Contribution to journalArticle

2 Citations (Scopus)
156 Downloads (Pure)

Abstract

Clostridium difficile is a spore forming bacterium and the leading cause of colitis and antibiotic associated diarrhoea in the developed world. Effective recovery of spores, particularly in low numbers, is imperative to obtain accurate prevalence data, due to the low number of spores found within non-clinical samples (<20/ml). Through comparison of C. difficile enrichment media, this study showed the importance of selecting an effective enrichment media. Commonly used broths, such as Cooked Meat broth, promote significantly less growth than other available broths such as Brain Heart Infusion broth, BHI. The optimization of BHI using selective antibiotics, moxalactam and norfloxacin, and sodium taurocholate at a concentration of 0.4%, allowed for high growth rate (0.465 hour-1), short lag times (<14 hours) and recovery of spores at low concentrations. The optimized broth, designated BHIMN-T, out-performed other commonly used broths so can be recommended for future studies.

Original languageEnglish
Pages (from-to)92-99
Number of pages8
JournalAnaerobe
Volume54
Early online date16 Aug 2018
DOIs
Publication statusPublished - Dec 2018

Fingerprint Dive into the research topics of 'Development of an optimized broth enrichment culture medium for the isolation of Clostridium difficile'. Together they form a unique fingerprint.

Cite this