Development of liposome-based freeze-dried rods for vaginal vaccine delivery against HIV-1

Prem N Gupta; Aditya Pattani; R Karl Malcolm; Rhonda M Curran; Gavin Andrews

doi:10.1016/j.jconrel.2010.07.081

Development of liposome-based freeze-dried rods for vaginal vaccine delivery against HIV-1

Prem N Gupta, Aditya Pattani, R Karl Malcolm, Rhonda M Curran, Gavin Andrews

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Abstract

The present investigation deals with development and characteriza- tion of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods, which were then characterized for moisture content. The redispersibility of the liposomes-based freeze- dried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection.

Original language	English
Pages (from-to)	e108-e110
Number of pages	3
Journal	Journal of Controlled Release
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/j.jconrel.2010.07.081
Publication status	Published - 19 Nov 2010

Keywords

AIDS Vaccines
Administration, Intravaginal
Animals
Drug Delivery Systems
Female
Freeze Drying
HIV-1
Immunization
Liposomes
Mice
Particle Size

ASJC Scopus subject areas

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Development of liposome-based freeze-dried rods for vaginal vaccine delivery against HIV-1",

abstract = "The present investigation deals with development and characteriza- tion of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods, which were then characterized for moisture content. The redispersibility of the liposomes-based freeze- dried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection.",

keywords = "AIDS Vaccines, Administration, Intravaginal, Animals, Drug Delivery Systems, Female, Freeze Drying, HIV-1, Immunization, Liposomes, Mice, Particle Size",

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AU - Pattani, Aditya

AU - Malcolm, R Karl

AU - Curran, Rhonda M

AU - Andrews, Gavin

PY - 2010/11/19

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AB - The present investigation deals with development and characteriza- tion of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods, which were then characterized for moisture content. The redispersibility of the liposomes-based freeze- dried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection.

KW - AIDS Vaccines

KW - Administration, Intravaginal

KW - Animals

KW - Drug Delivery Systems

KW - Female

KW - Freeze Drying

KW - HIV-1

KW - Immunization

KW - Liposomes

KW - Mice

KW - Particle Size

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Development of liposome-based freeze-dried rods for vaginal vaccine delivery against HIV-1

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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