Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery

Arvind K Jain, Ashley Massey, Helmy Yusuf, Denise M McDonald, Helen O McCarthy, Victoria L Kett

Research output: Contribution to journalArticle

9 Citations (Scopus)
321 Downloads (Pure)

Abstract

We report the formulation of novel composite nanoparticles that combine the high transfection efficiency of cationic peptide-DNA nanoparticles with the biocompatibility and prolonged delivery of polylactic acid–polyethylene glycol (PLA-PEG). The cationic cell-penetrating peptide RALA was used to condense DNA into nanoparticles that were encapsulated within a range of PLA-PEG copolymers. The composite nanoparticles produced exhibited excellent physicochemical properties including size <200 nm and encapsulation efficiency >80%. Images of the composite nanoparticles obtained with a new transmission electron microscopy staining method revealed the peptide-DNA nanoparticles within the PLA-PEG matrix. Varying the copolymers modulated the DNA release rate >6 weeks in vitro. The best formulation was selected and was able to transfect cells while maintaining viability. The effect of transferrin-appended composite nanoparticles was also studied. Thus, we have demonstrated the manufacture of composite nanoparticles for the controlled delivery of DNA.
Original languageEnglish
Pages (from-to)7183—7196
Number of pages14
JournalInternational Journal of Nanomedicine
Volume2015
Issue number10
DOIs
Publication statusPublished - 24 Nov 2015

Keywords

  • PLA-PEG, cationic peptide, gene delivery, composite nanoparticles, DNA, transfection

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