BackgroundAn early detection tool for EOC was constructed from analysis of biomarker expression data from serum collected during the UKCTOCS.MethodsThis study included 49 EOC cases (19 Type I and 30 Type II) and 31 controls, representing 482 serial samples spanning seven years pre-diagnosis. A logit model was trained by analysis of dysregulation of expression data of four putative biomarkers, (CA125, phosphatidylcholine-sterol acyltransferase, vitamin K-dependent protein Z and C-reactive protein); by scoring the specificity associated with dysregulation from the baseline expression for each individual.ResultsThe model is discriminatory, passes k-fold and leave-one-out cross-validations and was further validated in a Type I EOC set. Samples were analysed as a simulated annual screening programme, the algorithm diagnosed cases with >30% PPV 1–2 years pre-diagnosis. For Type II cases (~80% were HGS) the algorithm classified 64% at 1 year and 28% at 2 years tDx as severe.ConclusionsThe panel has the potential to diagnose EOC one-two years earlier than current diagnosis. This analysis provides a tangible worked example demonstrating the potential for development as a screening tool and scrutiny of its properties. Limits on interpretation imposed by the number of samples available are discussed.
Russell, M. R., Graham, C., D’Amato, A., Gentry-Maharaj, A., Ryan, A., Kalsi, J. K., ... Graham, R. L. J. (2019). Diagnosis of epithelial ovarian cancer using a combined protein biomarker panel. British Journal of Cancer. https://doi.org/10.1038/s41416-019-0544-0