Differential modulation of a radiation-induced bystander effect in glioblastoma cells by pifithrin-alpha and wortmannin

C.L. Shao, J.H. Zhang, Kevin Prise

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The implication of radiation-induced bystander effect (RIBE) for both radiation protection and radiotherapy has attracted significant attention, but a key question is how to modulate the RIBE. The present study found that, when a fraction of glioblastoma cells in T98G population were individually targeted with precise helium particles through their nucleus, micronucleus (MN) were induced and its yield increased non-linearly with radiation dose. After co-culturing with irradiated cells, additional MN could be induced in the non-irradiated bystander cells and its yield was independent of irradiation dose, giving direct evidence of a RIBE. Further results showed that the RIBE could be eliminated by pifithrin-alpha (p53 inhibitor) but enhanced by wortmannin (PI3K inhibitor). Moreover, it was found that nitric oxide (NO) contributed to this RIBE, and the levels of NO of both irradiated cells and bystander cells could be extensively diminished by pifithrin-alpha but insignificantly reduced by wortmannin. Our results indicate that RIBE can be modulated by p53 and PI3K through a NO-dependent and NO-independent pathway, respectively. (C) 2009 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)627-631
Number of pages5
JournalNuclear Instruments & Methods In Physics Research Section B-beam Interactions With Materials And Atoms
Volume268
Issue number6
DOIs
Publication statusPublished - 15 Mar 2010

ASJC Scopus subject areas

  • Instrumentation
  • Nuclear and High Energy Physics

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