Abstract
| Language | English |
|---|---|
| Title of host publication | Nature Neuroscience |
| Publisher | Nature Publishing Group |
| Pages | 1-9 |
| Number of pages | 9 |
| ISBN (Print) | 1546-1726 (Electronic)1097-6256 (Linking) |
| DOIs | |
| Publication status | Published - 18 Jun 2018 |
Publication series
| Name | Nature Neuroscience |
|---|
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Direct pericyte-to-neuron reprogramming via unfolding of a neural stem cell-like program. / Karow, Marisa; Camp, J. Gray; Falk, Sven; Gerber, Tobias; Pataskar, Abhijeet; Gac-Santel, Malgorzata; Kageyama, Jorge; Brazovskaja, Agnieska; Garding, Angela; Fan, Wenqiang; Riedemann, Therese; Casamassa, Antonella; Smiyakin, Andrej; Schichor, Christian; Götz, Magdalena; Tiwari, Vijay K.; Treutlein, Barbara; Berninger, Benedikt.
Nature Neuroscience. Nature Publishing Group, 2018. p. 1-9 (Nature Neuroscience).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Direct pericyte-to-neuron reprogramming via unfolding of a neural stem cell-like program
AU - Karow, Marisa
AU - Camp, J. Gray
AU - Falk, Sven
AU - Gerber, Tobias
AU - Pataskar, Abhijeet
AU - Gac-Santel, Malgorzata
AU - Kageyama, Jorge
AU - Brazovskaja, Agnieska
AU - Garding, Angela
AU - Fan, Wenqiang
AU - Riedemann, Therese
AU - Casamassa, Antonella
AU - Smiyakin, Andrej
AU - Schichor, Christian
AU - Götz, Magdalena
AU - Tiwari, Vijay K.
AU - Treutlein, Barbara
AU - Berninger, Benedikt
PY - 2018/6/18
Y1 - 2018/6/18
N2 - Ectopic expression of defined transcription factors can force direct cell-fate conversion from one lineage to another in the absence of cell division. Several transcription factor cocktails have enabled successful reprogramming of various somatic cell types into induced neurons (iNs) of distinct neurotransmitter phenotype. However, the nature of the intermediate states that drive the reprogramming trajectory toward distinct iN types is largely unknown. Here we show that successful direct reprogramming of adult human brain pericytes into functional iNs by Ascl1 and Sox2 encompasses transient activation of a neural stem cell-like gene expression program that precedes bifurcation into distinct neuronal lineages. During this transient state, key signaling components relevant for neural induction and neural stem cell maintenance are regulated by and functionally contribute to iN reprogramming and maturation. Thus, Ascl1- and Sox2-mediated reprogramming into a broad spectrum of iN types involves the unfolding of a developmental program via neural stem cell-like intermediates.
AB - Ectopic expression of defined transcription factors can force direct cell-fate conversion from one lineage to another in the absence of cell division. Several transcription factor cocktails have enabled successful reprogramming of various somatic cell types into induced neurons (iNs) of distinct neurotransmitter phenotype. However, the nature of the intermediate states that drive the reprogramming trajectory toward distinct iN types is largely unknown. Here we show that successful direct reprogramming of adult human brain pericytes into functional iNs by Ascl1 and Sox2 encompasses transient activation of a neural stem cell-like gene expression program that precedes bifurcation into distinct neuronal lineages. During this transient state, key signaling components relevant for neural induction and neural stem cell maintenance are regulated by and functionally contribute to iN reprogramming and maturation. Thus, Ascl1- and Sox2-mediated reprogramming into a broad spectrum of iN types involves the unfolding of a developmental program via neural stem cell-like intermediates.
UR - http://www.mendeley.com/research/direct-pericytetoneuron-reprogramming-via-unfolding-neural-stem-celllike-program
U2 - 10.1038/s41593-018-0168-3
DO - 10.1038/s41593-018-0168-3
M3 - Chapter
SN - 1546-1726 (Electronic)1097-6256 (Linking)
T3 - Nature Neuroscience
SP - 1
EP - 9
BT - Nature Neuroscience
PB - Nature Publishing Group
ER -