Distinctive effects of G-CSF, GM-CSF and TNFalpha on neutrophil apoptosis in systemic lupus erythematosus.

Aubrey Bell, D.J. Armstrong, E.M. Whitehead, A.D. Crockard

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

OBJECTIVE: To investigate the influence of culture with G-CSF GM-CSF and TNFalpha on neutrophil apoptosis, comparing neutrophils from SLE patients with rheumatoid arthritis (RA) patients and healthy control subjects. METHODS: Neutrophils were isolated from SLE (n= 10), RA (n= 10) and healthy control subjects (n= 10), and cultured with two different concentrations of G-CSF, GM-CSF and TNFalpha. Proportion of apoptotic neutrophils at T=0, T=2hrs and T=24hrs was measured using FITC-labelled annexinV and flow cytometry. RESULTS: Significantly more neutrophils were apoptotic at T=0 in the SLE subjects than in the other groups (median, range--Control 3.5% (0.3-7.9) SLE 9.5% (2.9-29.1) RA 3.0% (0.4-23.0) p
Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalClinical and Experimental Rheumatology
Volume23(2)
Publication statusPublished - Mar 2005

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Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Systemic Lupus Erythematosus
Neutrophils
Tumor Necrosis Factor-alpha
Apoptosis
Rheumatoid Arthritis
Healthy Volunteers
Fluorescein-5-isothiocyanate
Flow Cytometry

Cite this

Bell, Aubrey ; Armstrong, D.J. ; Whitehead, E.M. ; Crockard, A.D. / Distinctive effects of G-CSF, GM-CSF and TNFalpha on neutrophil apoptosis in systemic lupus erythematosus. In: Clinical and Experimental Rheumatology. 2005 ; Vol. 23(2). pp. 152-158.
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abstract = "OBJECTIVE: To investigate the influence of culture with G-CSF GM-CSF and TNFalpha on neutrophil apoptosis, comparing neutrophils from SLE patients with rheumatoid arthritis (RA) patients and healthy control subjects. METHODS: Neutrophils were isolated from SLE (n= 10), RA (n= 10) and healthy control subjects (n= 10), and cultured with two different concentrations of G-CSF, GM-CSF and TNFalpha. Proportion of apoptotic neutrophils at T=0, T=2hrs and T=24hrs was measured using FITC-labelled annexinV and flow cytometry. RESULTS: Significantly more neutrophils were apoptotic at T=0 in the SLE subjects than in the other groups (median, range--Control 3.5{\%} (0.3-7.9) SLE 9.5{\%} (2.9-29.1) RA 3.0{\%} (0.4-23.0) p",
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Distinctive effects of G-CSF, GM-CSF and TNFalpha on neutrophil apoptosis in systemic lupus erythematosus. / Bell, Aubrey; Armstrong, D.J.; Whitehead, E.M.; Crockard, A.D.

In: Clinical and Experimental Rheumatology, Vol. 23(2), 03.2005, p. 152-158.

Research output: Contribution to journalArticle

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T1 - Distinctive effects of G-CSF, GM-CSF and TNFalpha on neutrophil apoptosis in systemic lupus erythematosus.

AU - Bell, Aubrey

AU - Armstrong, D.J.

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AU - Crockard, A.D.

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AB - OBJECTIVE: To investigate the influence of culture with G-CSF GM-CSF and TNFalpha on neutrophil apoptosis, comparing neutrophils from SLE patients with rheumatoid arthritis (RA) patients and healthy control subjects. METHODS: Neutrophils were isolated from SLE (n= 10), RA (n= 10) and healthy control subjects (n= 10), and cultured with two different concentrations of G-CSF, GM-CSF and TNFalpha. Proportion of apoptotic neutrophils at T=0, T=2hrs and T=24hrs was measured using FITC-labelled annexinV and flow cytometry. RESULTS: Significantly more neutrophils were apoptotic at T=0 in the SLE subjects than in the other groups (median, range--Control 3.5% (0.3-7.9) SLE 9.5% (2.9-29.1) RA 3.0% (0.4-23.0) p

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