A fast screening method was developed to assess the pathogenicity of a diverse collection of environmental and clinical Burkholderia cepacia complex isolates in the nematode Caenorhabditis elegans. The method was validated by comparison with the standard slow-killing assay. We observed that the pathogenicity of B. cepacia complex isolates in C. elegans was strain-dependent but species-independent. The wide range of observed pathogenic phenotypes agrees with the high degree of phenotypic variation among species of the B. cepacia complex and suggests that the taxonomic classification of a given strain within the complex cannot predict pathogenicity.
ASJC Scopus subject areas
- Molecular Biology
- Applied Microbiology and Biotechnology
Cardona, S. T., Wopperer, J., Eberl, L., & Valvano, M. A. (2005). Diverse pathogenicity of Burkholderia cepacia complex strains in the Caenorhabditis elegans host model. FEMS Microbiology Letters, 250(1), 97-104. https://doi.org/10.1016/j.femsle.2005.06.050