Diverse pathogenicity of Burkholderia cepacia complex strains in the Caenorhabditis elegans host model

Silvia T Cardona; Julia Wopperer; Leo Eberl; Miguel A Valvano

doi:10.1016/j.femsle.2005.06.050

Diverse pathogenicity of Burkholderia cepacia complex strains in the Caenorhabditis elegans host model

Silvia T Cardona, Julia Wopperer, Leo Eberl, Miguel A Valvano

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

A fast screening method was developed to assess the pathogenicity of a diverse collection of environmental and clinical Burkholderia cepacia complex isolates in the nematode Caenorhabditis elegans. The method was validated by comparison with the standard slow-killing assay. We observed that the pathogenicity of B. cepacia complex isolates in C. elegans was strain-dependent but species-independent. The wide range of observed pathogenic phenotypes agrees with the high degree of phenotypic variation among species of the B. cepacia complex and suggests that the taxonomic classification of a given strain within the complex cannot predict pathogenicity.

Original language	English
Pages (from-to)	97-104
Number of pages	8
Journal	FEMS Microbiology Letters
Volume	250
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2005.06.050
Publication status	Published - 01 Sept 2005

ASJC Scopus subject areas

Genetics
Molecular Biology
Applied Microbiology and Biotechnology
Microbiology

Access to Document

10.1016/j.femsle.2005.06.050

Cite this

@article{65d0cd9af1e346fdb865cd069c1ca45e,

title = "Diverse pathogenicity of Burkholderia cepacia complex strains in the Caenorhabditis elegans host model",

abstract = "A fast screening method was developed to assess the pathogenicity of a diverse collection of environmental and clinical Burkholderia cepacia complex isolates in the nematode Caenorhabditis elegans. The method was validated by comparison with the standard slow-killing assay. We observed that the pathogenicity of B. cepacia complex isolates in C. elegans was strain-dependent but species-independent. The wide range of observed pathogenic phenotypes agrees with the high degree of phenotypic variation among species of the B. cepacia complex and suggests that the taxonomic classification of a given strain within the complex cannot predict pathogenicity.",

author = "Cardona, {Silvia T} and Julia Wopperer and Leo Eberl and Valvano, {Miguel A}",

year = "2005",

month = sep,

day = "1",

doi = "10.1016/j.femsle.2005.06.050",

language = "English",

volume = "250",

pages = "97--104",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Diverse pathogenicity of Burkholderia cepacia complex strains in the Caenorhabditis elegans host model

AU - Cardona, Silvia T

AU - Wopperer, Julia

AU - Eberl, Leo

AU - Valvano, Miguel A

PY - 2005/9/1

Y1 - 2005/9/1

N2 - A fast screening method was developed to assess the pathogenicity of a diverse collection of environmental and clinical Burkholderia cepacia complex isolates in the nematode Caenorhabditis elegans. The method was validated by comparison with the standard slow-killing assay. We observed that the pathogenicity of B. cepacia complex isolates in C. elegans was strain-dependent but species-independent. The wide range of observed pathogenic phenotypes agrees with the high degree of phenotypic variation among species of the B. cepacia complex and suggests that the taxonomic classification of a given strain within the complex cannot predict pathogenicity.

AB - A fast screening method was developed to assess the pathogenicity of a diverse collection of environmental and clinical Burkholderia cepacia complex isolates in the nematode Caenorhabditis elegans. The method was validated by comparison with the standard slow-killing assay. We observed that the pathogenicity of B. cepacia complex isolates in C. elegans was strain-dependent but species-independent. The wide range of observed pathogenic phenotypes agrees with the high degree of phenotypic variation among species of the B. cepacia complex and suggests that the taxonomic classification of a given strain within the complex cannot predict pathogenicity.

U2 - 10.1016/j.femsle.2005.06.050

DO - 10.1016/j.femsle.2005.06.050

M3 - Article

C2 - 16043310

SN - 0378-1097

VL - 250

SP - 97

EP - 104

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Diverse pathogenicity of Burkholderia cepacia complex strains in the Caenorhabditis elegans host model

Abstract

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this