Down-regulation of PERK enhances resistance to ionizing radiation

Deepu Oommen*, Kevin M. Prise

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)
23 Downloads (Pure)

Abstract

Although, ionizing radiation (IR) has been implicated to cause stress in endoplasmic reticulum (ER), how ER stress signaling and major ER stress sensors modulate cellular response to IR is unclear. Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an ER transmembrane protein which initiates unfolded protein response (UPR) or ER stress signaling when ER homeostasis is disturbed. Here, we report that down-regulation of PERK resulted in increased clonogenic survival, enhanced DNA repair and reduced apoptosis in irradiated cancer cells. Our study demonstrated that PERK has a role in sensitizing cancer cells to IR. 

Original languageEnglish
Pages (from-to)31-35
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume441
Issue number1
Early online date05 Oct 2013
DOIs
Publication statusPublished - 08 Nov 2013

Keywords

  • PERK
  • Ionizing radiation
  • DNA damage
  • ER-stress
  • ENDOPLASMIC-RETICULUM STRESS
  • UNFOLDED PROTEIN RESPONSE
  • INDUCED APOPTOSIS
  • SIGNALING PATHWAYS
  • DNA-DAMAGE
  • MAMMALIAN-CELLS
  • BREAST-CANCER
  • TRANSLATION
  • KINASE
  • PHOSPHORYLATION

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