Droplet Digital PCR Diagnosis of Human Schistosomiasis Parasite Cell-Free DNA Detection in Diverse Clinical Samples

Kosala G. Weerakoon*, Catherine A. Gordon, Gail M. Williams, Pengfei Cai, Geoffrey N. Gobert, Remigio M. Olveda, Allen G. Ross, David U. Olveda, Donald P. McManus

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Background

Schistosomiasis japonica remains a major public health and socioeconomic concern in Southeast Asia. Sensitive and accurate diagnostics can play a pivotal role in achieving disease elimination goals.

Methods

We previously reported a novel droplet digital polymerase chain reaction (ddPCR) assay targeting the mitochondrial gene nad1 to diagnose schistosomiasis japonica. The tool identified both prepatent and patent infections using Schistosoma japonicum DNA isolated from serum, urine, salivary glands, and feces in a murine model. The assay was validated here using clinical samples collected from 412 subjects resident in an area moderately endemic for schistosomiasis in the Philippines. 

Results

S. japonicum DNA present in human stool, serum, urine, and saliva was detected quantitatively with high sensitivity. The capability to diagnose cases of human schistosomiasis using noninvasively collected clinical samples, the higher level of sensitivity obtained compared with the microscopy-based Kato-Katz test, and the capacity to quantify infection intensity have important public health implications for schistosomiasis control and programs targeting other neglected tropical diseases. Conclusions. This verified ddPCR method represents a valuable new tool for the diagnosis and surveillance of schistosomiasis, particularly in low-prevalence and low-intensity areas approaching elimination and in monitoring where disease emergence or re-emergence is a concern.

Original languageEnglish
Pages (from-to)1611-1622
JournalJournal of Infectious Diseases
Volume216
Issue number12
Early online date27 Sep 2017
DOIs
Publication statusPublished - 15 Dec 2017

Keywords

  • diagnostics
  • droplet digital PCR
  • Schistosoma japonicum
  • schistosomiasis
  • surveillance

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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