Dual-colour HER2/chromosome 17 chromogenic in situ hybridisation assay enables accurate assessment of HER2 genomic status in gastric cancer and has potential utility in HER2 testing of biopsy samples

  • Benedict Yan
  • , Ee Xuan Yau
  • , Shoa Nian Choo
  • , Chee Wee Ong
  • , Kol Jia Yong
  • , Brendan Pang
  • , Manuel Salto-Tellez

Research output: Contribution to journalArticlepeer-review

Abstract

Determination of HER2 protein expression by immunohistochemistry (IHC) and genomic status by fluorescent in situ hybridisation (FISH) are important in identifying a subset of high HER2-expressing gastric cancers that might respond to trastuzumab. Although FISH is considered the standard for determination of HER2 genomic status, brightfield ISH is being increasingly recognised as a viable alternative. Also, the impact of HER2 protein expression/genomic heterogeneity on the accuracy of HER2 testing has not been well studied in the context of gastric biopsy samples.
Original languageEnglish
Pages (from-to)880-3
Number of pages4
JournalJournal of Clinical Pathology
Volume64
Issue number10
DOIs
Publication statusPublished - Oct 2011

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • Chromosomes, Human, Pair 17
  • Receptor, erbB-2
  • Chi-Square Distribution
  • Humans
  • In Situ Hybridization, Fluorescence
  • Biopsy
  • Predictive Value of Tests
  • Gene Amplification
  • False Negative Reactions
  • In Situ Hybridization
  • Stomach Neoplasms
  • Singapore
  • Tumor Markers, Biological
  • Immunohistochemistry

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