Dynamics of chromatin accessibility and epigenetic state in response to UV damage

Sandra Schick, David Fournier, Sudhir Thakurela, Sanjeeb Kumar Sahu, Angela Garding, Vijay K Tiwari

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Epigenetic mechanisms determine the access of regulatory factors to DNA during events such as transcription and the DNA damage response. However, the global response of histone modifications and chromatin accessibility to UV exposure remains poorly understood. Here, we report that UV exposure results in a genome-wide reduction in chromatin accessibility, while the distribution of the active regulatory mark H3K27ac undergoes massive reorganization. Genomic loci subjected to epigenetic reprogramming upon UV exposure represent target sites for sequence-specific transcription factors. Most of these are distal regulatory regions, highlighting their importance in the cellular response to UV exposure. Furthermore, UV exposure results in an extensive reorganization of super-enhancers, accompanied by expression changes of associated genes, which may in part contribute to the stress response. Taken together, our study provides the first comprehensive resource for genome-wide chromatin changes upon UV irradiation in relation to gene expression and elucidates new aspects of this relationship.

Original languageEnglish
Pages (from-to)4380-94
Number of pages15
JournalJournal of Cell Science
Volume128
Issue number23
DOIs
Publication statusPublished - 01 Dec 2015
Externally publishedYes

Bibliographical note

© 2015. Published by The Company of Biologists Ltd.

Keywords

  • Animals
  • Chromatin/genetics
  • Chromatin Assembly and Disassembly/radiation effects
  • DNA Damage
  • Epigenesis, Genetic/radiation effects
  • Mice
  • NIH 3T3 Cells
  • Ultraviolet Rays/adverse effects

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