Dynamics of Viral RNA Synthesis during Measles Virus Infection

S. Plumet, Paul Duprex, D. Gerlier

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

We propose a reference model of the kinetics of a viral RNA-dependent RNA polymerase (vRdRp) activities and its regulation during infection of eucaryotic cells. After measles virus infects a cell, mRNAs from all genes immediately start to accumulate linearly over the first 5 to 6 h and then exponentially until approximately 24 h. The change from a linear to an exponential accumulation correlates with de novo synthesis of vRdRp from the incoming template. Expression of the virus nucleoprotein (N) prior to infection shifts the balance in favor of replication. Conversely, inhibition of protein synthesis by cycloheximide favors the latter. The in vivo elongation speed of the viral polymerase is approximately 3 nucleotides/s. A similar profile with fivefold-slower kinetics can be obtained using a recombinant virus expressing a structurally altered polymerase. Finally, virions contain only encapsidated genomic, antigenomic, and 5'-end abortive replication fragment RNAs.
Original languageEnglish
Pages (from-to)6900-6908
Number of pages9
JournalJournal of Virology
Volume79(11)
Issue number11
DOIs
Publication statusPublished - Jun 2005

ASJC Scopus subject areas

  • Immunology

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