Early detection of leukaemic relapse after bone marrow transplantation using the polymerase chain reaction

P V Browne, M Lawler, J O'Riordan, P Humphries, S R McCann, Mark Lawler

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We report a case of acute lymphoblastic leukaemia relapsing after allogeneic bone marrow transplantation in which the polymerase chain reaction (PCR) was used to assess chimeric status. This technique demonstrated the progressive reappearance of host cells prior to clinical relapse. The relapse was of host cell origin as shown by the presence of female (recipient) metaphases containing an abnormal chromosomal marker (iso 9q) which had also been present at initial diagnosis. The emergence of host cells in this case, detected only by PCR techniques but not by cytogenetic methods, appeared to herald overt relapse. PCR analysis provides a sensitive tool for detecting a progressive rise in host cell numbers which may predict clinical relapse.

Original languageEnglish
Pages (from-to)167-9
Number of pages3
JournalBone Marrow Transplantation
Volume7
Issue number2
Publication statusPublished - Feb 1991

Keywords

  • Adult
  • Bone Marrow Transplantation
  • Cell Cycle
  • Chimera
  • Chromosomes
  • DNA
  • Female
  • Graft Rejection
  • Hematopoietic Stem Cells
  • Humans
  • Karyotyping
  • Metaphase
  • Polymerase Chain Reaction
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma

Fingerprint

Dive into the research topics of 'Early detection of leukaemic relapse after bone marrow transplantation using the polymerase chain reaction'. Together they form a unique fingerprint.

Cite this