Early onset of changes to the reproductive system of Fasciola hepatica following in vivo treatment with triclabendazole

R.E.B. Hanna, S. Scarcella, H. Solana, S McConnell, Ian Fairweather

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Lambs infected with the Cullompton triclabendazole (ICBZ)-susceptible isolate of Fasciola hepatica were treated with TCBZ at a dosage of 10 mg/kg at 16 weeks post-infection. Adult flukes were recovered from the liver at 3 h, 24 h, 48 h and 60 h post-treatment (pt). They were processed for histological analysis of the uterus, Mehlis' gland, vitellaria, ovary and testis. At 3 h pt, the flukes were essentially similar to the controls and were producing normal eggs. Egg production had ceased by 24 h pt. At this time period, the cells of the Mehlis' gland showed some evidence of vacuolation, but otherwise were relatively normal. A shift in the population of vitelline cells towards mature cells was observed at 24 h pt, and this trend continued at later time-periods. It was accompanied by a breakdown of the cells and the presence of apoptotic bodies. Marked changes to the ovary were first noted at 48 h Pt, as evidenced by vacuolation and the presence of apoptotic bodies. Some disruption to the testis was seen at 24 h pt, with a reduction in the population of spermatogenic cells, the appearance of apoptotic bodies and some peripheral vacuolation of the tubules. These abnormalities increased in severity with longer time periods pt. The results bring forward the time-line of cessation of egg production by 24 h, demonstrating that this process is affected very rapidly pt.
Original languageEnglish
Pages (from-to)341-347
Number of pages7
JournalVeterinary Parasitology
Volume184
Issue number2-4
Early online date19 Aug 2011
DOIs
Publication statusPublished - 23 Mar 2012

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Fingerprint Dive into the research topics of 'Early onset of changes to the reproductive system of Fasciola hepatica following in vivo treatment with triclabendazole'. Together they form a unique fingerprint.

  • Cite this