ECFS CTN SOP sputum induction for expectorating older children and adults

Kathryn Hill, Oriane Burgen, Marie Dautry, Jana Pleskova, Anna Chelmerova, Isabelle Sermet-Gaudelus

Research output: Other contributionProtocolpeer-review


Goal of Sputum Induction Procedure:
To obtain an adequate sputum sample, the minimum acceptable total volume for an induced sputum sample is advised to be 0.5mL per collection container. If only clear liquid (with no clumps/plugs) is visible, an inadequate quantity of sputum may have been collected.

The SI procedure consists of:
• Administration of the participant’s prescribed bronchodilator, if a bronchodilator is not usually prescribed Salamol MDI should be prescribed and administered.
• Four, 3-minute cycles (with an optional 5th cycle) during which the participant breathes aerosolized 3% hypertonic saline (HTS) and then expectorates sputum into a sample container (discuss the % HTS used on a case-by-case basis if needed i.e., if the participant uses 7% HTS as part of their airway clearance, this should be noted. If they are or have been prescribed 7% HTS, giving them 3% may not work).
• An airway clearance technique (ACT) will be taught and/or administered by a physiotherapist trained in ACT, to aid secretion expectoration.
• Sputum is collected by spontaneous expectoration if the participant can cooperate.
• The entire sputum production procedure takes approximately 25-35 minutes.
• If the participant cannot expectorate, see separate ECFS-CTN SOP for Non-Expectorating Older Children and Adults ECFS-CTN/2.4/001/04.

Original languageEnglish
TypeECFS CTN SOP Sputum Induction for Expectorating Older Children and Adults
Media of outputECFS Education Platform: electronic
PublisherEuropean Cystic Fibrosis Society
Number of pages24
Publication statusPublished - 17 Apr 2024


  • sputum induction
  • cystic fibrosis
  • children
  • adults

ASJC Scopus subject areas

  • Medicine(all)


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