Abstract
The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm(2) using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm(2) on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.
| Original language | English |
|---|---|
| Pages (from-to) | 33-9 |
| Number of pages | 7 |
| Journal | Lasers in medical science |
| Volume | 25 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2010 |
Keywords
- Adenosine Triphosphate/metabolism
- Adipose Tissue/cytology
- Adult Stem Cells/cytology
- Biomarkers/metabolism
- Cell Proliferation/drug effects
- Cell Survival/drug effects
- Cells, Cultured
- Epidermal Growth Factor/pharmacology
- Humans
- Lasers, Semiconductor/therapeutic use
- Low-Level Light Therapy
- Luminescence
- Thy-1 Antigens/metabolism