Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells

B Mvula, T J Moore, H Abrahamse

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm(2) using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm(2) on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.

Original languageEnglish
Pages (from-to)33-9
Number of pages7
JournalLasers in medical science
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 2010

Keywords

  • Adenosine Triphosphate/metabolism
  • Adipose Tissue/cytology
  • Adult Stem Cells/cytology
  • Biomarkers/metabolism
  • Cell Proliferation/drug effects
  • Cell Survival/drug effects
  • Cells, Cultured
  • Epidermal Growth Factor/pharmacology
  • Humans
  • Lasers, Semiconductor/therapeutic use
  • Low-Level Light Therapy
  • Luminescence
  • Thy-1 Antigens/metabolism

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