Effect of microneedle treatment on the skin permeation of a nanoencapsulated dye

Yasmine A. Gomaa, Labiba K. El-Khordagui*, Martin J. Garland, Ryan F. Donnelly, Fiona McInnes, Victor M. Meidan

*Corresponding author for this work

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Objectives The aim of the study was to investigate the effect of microneedle (MN) pretreatment on the transdermal delivery of a model drug (Rhodamine B, Rh B) encapsulated in polylactic-co-glycolic acid (PLGA) nanoparticles (NPs) focusing on the MN characteristics and application variables. Methods Gantrez MNs were fabricated using laser-engineered silicone micro-mould templates. PLGA NPs were prepared using a modified emulsion-diffusion-evaporation method and characterised in vitro. Permeation of encapsulated Rh B through MN-treated full thickness porcine skin was performed using Franz diffusion cells with appropriate controls. Key findings In-vitro skin permeation of the nanoencapsulated Rh B (6.19 ± 0.77 μg/cm 2/h) was significantly higher (P < 0.05) compared with the free solution (1.66 ± 0.53 μg/cm2/h). Mechanistic insights were supportive of preferential and rapid deposition of NPs in the MN-created microconduits, resulting in accelerated dye permeation. Variables such as MN array configuration and application mode were shown to affect transdermal delivery of the nanoencapsulated dye. Conclusions This dual MN/NP-mediated approach offers potential for both the dermal and transdermal delivery of therapeutic agents with poor passive diffusion characteristics.

Original languageEnglish
Pages (from-to)1592-1602
Number of pages11
JournalJournal of Pharmacy and Pharmacology
Volume64
Issue number11
DOIs
Publication statusPublished - 01 Nov 2012

Keywords

  • microneedles
  • nanoparticles
  • Rhodamine B

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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