Effect of Paclitaxel-Loaded PLGA Nanoparticles on MDA-MB Type Cell Lines; Apoptosis and Cytotoxicity Studies

Venugopal Vijayan*, Krishnan Shalini, V. Yugesvaran, Teh Hui Yee, Siventhiran Balakrishnan, Vasanth Raj Palanimuthu

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Triple-Negative Breast Cancer is an aggressive type of breast cancer, which is not treatable by chemotherapy drugs, due to the lack of Estrogen Receptor (ER), Progesterone Receptor (PR) expression and Human Epidermal Growth Factor Receptor 2 (HER2) on the cell surface. 

Objective: The aim of this study was to compare the effect of paclitaxel loaded PLGA nanoparticle (PTX-NPs) on the cytotoxicity and apoptosis of the different MDA-MB type of cell lines. 

Method: PTX-NPs were prepared by nanoprecipitation method and characterized earlier. The cytotoxicity of PTX-NPs was evaluated by MTT and LDH assay, later apoptosis was calculated by flow cytometry analysis. 

Results: The prepared NP size of 317.5 nm and zetapontial of-12.7 mV showed drug release of 89.1 % at 48 h. MDA-MB-231 type cell showed significant cytotoxicity by MTT method of 47.4 ± 1.2 % at 24 h, 34.6 ± 0.8 % at 48 h and 23.5 ± 0.5 % at 72 h and LDH method of 35.9 ± 1.5 % at 24 h, 25.4 ± 0.6 % at 48 h and 19.8 ± 2.2 % at 72 h with apoptosis of 47.3 ± 0.4 %. 

Conclusion: We have found that PTX-NPs showed the cytotoxic effect on all the MDA-MB cancer cell lines and showed potent anticancer activities against MDA-MB-231 cell line via induction of apoptosis.

Original languageEnglish
Pages (from-to)3366-3375
Number of pages10
JournalCurrent Pharmaceutical Design
Volume24
Issue number28
DOIs
Publication statusPublished - 01 Oct 2018

Keywords

  • Apoptosis
  • MDA-MB
  • Nanoparticle
  • Paclitaxel
  • cell lines

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Fingerprint Dive into the research topics of 'Effect of Paclitaxel-Loaded PLGA Nanoparticles on MDA-MB Type Cell Lines; Apoptosis and Cytotoxicity Studies'. Together they form a unique fingerprint.

Cite this