Effects of modified low-density lipoproteins on human retinal pericyte survival

Weiwei Song; Jeremy L Barth; Kangmo Lu; Yongxin Yu; Yan Huang; Cynthia K Gittinger; W Scott Argraves; Timothy J Lyons

doi:10.1196/annals.1333.045

Effects of modified low-density lipoproteins on human retinal pericyte survival

Weiwei Song, Jeremy L Barth, Kangmo Lu, Yongxin Yu, Yan Huang, Cynthia K Gittinger, W Scott Argraves, Timothy J Lyons

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

According to a current paradigm cardiovascular diseases can be initiated by exposure of vascular cells to qualitatively modified low-density lipoproteins (LDL). Capillary leakage, an early feature of diabetic retinopathy, results in the exposure of retinal pericytes to modified LDL, including glycated (G-LDL) and heavily oxidized glycated LDL (HOG-LDL). We demonstrate here that modified LDL inhibits the proliferation and survival of cultured human retinal pericytes. Modified LDL also induced DNA fragmentation in bovine retinal pericytes. Overall, HOG-LDL produced a significantly higher extent of cytotoxicity and apoptosis in retinal pericytes. These results indicate that exposure of pericytes to HOG-LDL could be implicated in the development of diabetic retinopathy.

Original language	English
Pages (from-to)	390-5
Number of pages	6
Journal	Annals of the New York Academy of Sciences
Volume	1043
DOIs	https://doi.org/10.1196/annals.1333.045
Publication status	Published - Jun 2005

Keywords

Apoptosis
Cell Survival
DNA Fragmentation
Humans
Lipoproteins, LDL
Pericytes
Retina

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1196/annals.1333.045

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title = "Effects of modified low-density lipoproteins on human retinal pericyte survival",

abstract = "According to a current paradigm cardiovascular diseases can be initiated by exposure of vascular cells to qualitatively modified low-density lipoproteins (LDL). Capillary leakage, an early feature of diabetic retinopathy, results in the exposure of retinal pericytes to modified LDL, including glycated (G-LDL) and heavily oxidized glycated LDL (HOG-LDL). We demonstrate here that modified LDL inhibits the proliferation and survival of cultured human retinal pericytes. Modified LDL also induced DNA fragmentation in bovine retinal pericytes. Overall, HOG-LDL produced a significantly higher extent of cytotoxicity and apoptosis in retinal pericytes. These results indicate that exposure of pericytes to HOG-LDL could be implicated in the development of diabetic retinopathy.",

keywords = "Apoptosis, Cell Survival, DNA Fragmentation, Humans, Lipoproteins, LDL, Pericytes, Retina",

author = "Weiwei Song and Barth, {Jeremy L} and Kangmo Lu and Yongxin Yu and Yan Huang and Gittinger, {Cynthia K} and Argraves, {W Scott} and Lyons, {Timothy J}",

year = "2005",

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doi = "10.1196/annals.1333.045",

language = "English",

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T1 - Effects of modified low-density lipoproteins on human retinal pericyte survival

AU - Song, Weiwei

AU - Barth, Jeremy L

AU - Lu, Kangmo

AU - Yu, Yongxin

AU - Huang, Yan

AU - Gittinger, Cynthia K

AU - Argraves, W Scott

AU - Lyons, Timothy J

PY - 2005/6

Y1 - 2005/6

N2 - According to a current paradigm cardiovascular diseases can be initiated by exposure of vascular cells to qualitatively modified low-density lipoproteins (LDL). Capillary leakage, an early feature of diabetic retinopathy, results in the exposure of retinal pericytes to modified LDL, including glycated (G-LDL) and heavily oxidized glycated LDL (HOG-LDL). We demonstrate here that modified LDL inhibits the proliferation and survival of cultured human retinal pericytes. Modified LDL also induced DNA fragmentation in bovine retinal pericytes. Overall, HOG-LDL produced a significantly higher extent of cytotoxicity and apoptosis in retinal pericytes. These results indicate that exposure of pericytes to HOG-LDL could be implicated in the development of diabetic retinopathy.

AB - According to a current paradigm cardiovascular diseases can be initiated by exposure of vascular cells to qualitatively modified low-density lipoproteins (LDL). Capillary leakage, an early feature of diabetic retinopathy, results in the exposure of retinal pericytes to modified LDL, including glycated (G-LDL) and heavily oxidized glycated LDL (HOG-LDL). We demonstrate here that modified LDL inhibits the proliferation and survival of cultured human retinal pericytes. Modified LDL also induced DNA fragmentation in bovine retinal pericytes. Overall, HOG-LDL produced a significantly higher extent of cytotoxicity and apoptosis in retinal pericytes. These results indicate that exposure of pericytes to HOG-LDL could be implicated in the development of diabetic retinopathy.

KW - Apoptosis

KW - Cell Survival

KW - DNA Fragmentation

KW - Humans

KW - Lipoproteins, LDL

KW - Pericytes

KW - Retina

U2 - 10.1196/annals.1333.045

DO - 10.1196/annals.1333.045

M3 - Article

C2 - 16037260

VL - 1043

SP - 390

EP - 395

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 1749-6632

ER -

Effects of modified low-density lipoproteins on human retinal pericyte survival

Abstract

Keywords

UN SDGs

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