Efficient differentiation into skin cells of bone marrow cells recovered in a pellet after density gradient fractionation

Reinhold J Medina, Ken Kataoka, Masahiro Miyazaki, Nam-Ho Huh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We had previously demonstrated the participation of whole bone marrow cells from adult mice in the reconstitution of skin, including the epidermis and hair follicles. To get an insight into cell populations that give rise to the epithelial components of the reconstituted skin, we fractionated bone marrow cells derived from green fluorescent protein-transgenic mice by density gradient. Unexpectedly, we found that a substantial amount of mononucleated cells (approximately 30%) was recovered in the pellet fraction and that the cells in the pellet fraction preferentially differentiated into epithelial components of skin, rather than the cells in the mononuclear cell fraction. The pellet fraction contained more CD45-negative (thus uncommitted to the hematopoietic cell lineage) cells than the mononuclear cell fraction. These results indicate that density gradient fractionation results in significant loss of specific progenitor cells into the usually discarded pellet fraction.
Original languageEnglish
Pages (from-to)721-7
Number of pages7
JournalInternational Journal of Molecular Medicine
Volume17
Issue number5
Publication statusPublished - May 2006

ASJC Scopus subject areas

  • Genetics

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