Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering

John G. Hardy, R. Chase Cornelison, Rushi C. Sukhavasi, Richard J. Saballos, Philip Vu, David L. Kaplan, Christine E. Schmidt

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)
281 Downloads (Pure)

Abstract

Tissues in the body are hierarchically structured composite materials with tissue-specific chemical and topographical properties. Here we report the preparation of tissue scaffolds with macroscopic pores generated via the dissolution of a sacrificial supramolecular polymer-based crystal template (urea) from a biodegradable polymer-based scaffold (polycaprolactone, PCL). Furthermore, we report a method of aligning the supramolecular polymer-based crystals within the PCL, and that the dissolution of the sacrificial urea yields scaffolds with macroscopic pores that are aligned over long, clinically-relevant distances (i.e., centimeter scale). The pores act as topographical cues to which rat Schwann cells respond by aligning with the long axis of the pores. Generation of an interpenetrating network of polypyrrole (PPy) and poly(styrene sulfonate) (PSS) in the scaffolds yields electroactive tissue scaffolds that allow the electrical stimulation of Schwann cells cultured on the scaffolds which increases the production of nerve growth factor (NGF).
Original languageEnglish
Pages (from-to)15-34
Number of pages20
JournalBioengineering
Volume2
Issue number1
DOIs
Publication statusPublished - 14 Jan 2015
Externally publishedYes

Keywords

  • BIOMATERIALS
  • POROSITY
  • tissue scaffold
  • tissue engineering
  • Regenerative Medicine

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomaterials

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