Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells

Tatiana E Salazar; Matthew R Richardson; Eleni Beli; Matthew S Ripsch; John George; Youngsook Kim; Yaqian Duan; Leni Moldovan; Yuanqing Yan; Ashay Bhatwadekar; Vaishnavi Jadhav; Jared A Smith; Susan McGorray; Alicia L Bertone; Dmitri O Traktuev; Keith L March; Luis M Colon-Perez; Keith G Avin; Emily Sims; Julie A Mund; Jamie Case; Xiaolin Deng; Min Su Kim; Bruce McDavitt; Michael E Boulton; Jeffrey Thinschmidt; Sergio Li Calzi; Stephanie D Fitz; Robyn K Fuchs; Stuart J Warden; Todd McKinley; Anantha Shekhar; Marcelo Febo; Phillip L Johnson; Lung-Ji Chang; Zhanguo Gao; Mikhail G Kolonin; Song Lai; Jingfeng Ma; Xinzhong Dong; Fletcher A White; Huisheng Xie; Mervin C Yoder; Maria B Grant

doi:10.1002/stem.2613

Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells

Tatiana E Salazar, Matthew R Richardson, Eleni Beli, Matthew S Ripsch, John George, Youngsook Kim, Yaqian Duan, Leni Moldovan, Yuanqing Yan, Ashay Bhatwadekar, Vaishnavi Jadhav, Jared A Smith, Susan McGorray, Alicia L Bertone, Dmitri O Traktuev, Keith L March, Luis M Colon-Perez, Keith G Avin, Emily Sims, Julie A MundJamie Case, Xiaolin Deng, Min Su Kim, Bruce McDavitt, Michael E Boulton, Jeffrey Thinschmidt, Sergio Li Calzi, Stephanie D Fitz, Robyn K Fuchs, Stuart J Warden, Todd McKinley, Anantha Shekhar, Marcelo Febo, Phillip L Johnson, Lung-Ji Chang, Zhanguo Gao, Mikhail G Kolonin, Song Lai, Jingfeng Ma, Xinzhong Dong, Fletcher A White, Huisheng Xie, Mervin C Yoder, Maria B Grant

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Electroacupuncture (EA) performed in rats and humans using limb acupuncture sites, LI-4 and LI-11, and GV-14 and GV-20 (humans) and Bai-hui (rats) increased functional connectivity between the anterior hypothalamus and the amygdala and mobilized mesenchymal stem cells (MSCs) into the systemic circulation. In human subjects, the source of the MSC was found to be primarily adipose tissue, whereas in rodents the tissue sources were considered more heterogeneous. Pharmacological disinhibition of rat hypothalamus enhanced sympathetic nervous system (SNS) activation and similarly resulted in a release of MSC into the circulation. EA-mediated SNS activation was further supported by browning of white adipose tissue in rats. EA treatment of rats undergoing partial rupture of the Achilles tendon resulted in reduced mechanical hyperalgesia, increased serum interleukin-10 levels and tendon remodeling, effects blocked in propranolol-treated rodents. To distinguish the afferent role of the peripheral nervous system, phosphoinositide-interacting regulator of transient receptor potential channels (Pirt)-GCaMP3 (genetically encoded calcium sensor) mice were treated with EA acupuncture points, ST-36 and LIV-3, and GV-14 and Bai-hui and resulted in a rapid activation of primary sensory neurons. EA activated sensory ganglia and SNS centers to mediate the release of MSC that can enhance tissue repair, increase anti-inflammatory cytokine production and provide pronounced analgesic relief. Stem Cells 2017;35:1303-1315.

Original language	English
Pages (from-to)	1303-1315
Number of pages	13
Journal	Stem Cells
Volume	35
Issue number	5
Early online date	16 Mar 2017
DOIs	https://doi.org/10.1002/stem.2613
Publication status	Published - May 2017
Externally published	Yes

Bibliographical note

Keywords

Achilles Tendon/pathology
Acupuncture Points
Adipocytes/cytology
Adipose Tissue, Brown/cytology
Adipose Tissue, White/cytology
Animals
Antigens, CD/metabolism
Central Nervous System/cytology
Electroacupuncture
Forelimb/physiology
Hindlimb/physiology
Humans
Hyperalgesia/therapy
Hypothalamus/cytology
Interleukin-10/blood
Macrophages/cytology
Mesenchymal Stem Cells/cytology
Mice
Nerve Net/physiology
Rats
Rupture
Sensory Receptor Cells/metabolism
Uncoupling Protein 1/metabolism

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Salazar, T. E., Richardson, M. R., Beli, E., Ripsch, M. S., George, J., Kim, Y., Duan, Y., Moldovan, L., Yan, Y., Bhatwadekar, A., Jadhav, V., Smith, J. A., McGorray, S., Bertone, A. L., Traktuev, D. O., March, K. L., Colon-Perez, L. M., Avin, K. G., Sims, E., ... Grant, M. B. (2017). Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells. Stem Cells, 35(5), 1303-1315. https://doi.org/10.1002/stem.2613

@article{0b064a691efd4a6ea1c27cc5a3574aba,

title = "Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells",

abstract = "Electroacupuncture (EA) performed in rats and humans using limb acupuncture sites, LI-4 and LI-11, and GV-14 and GV-20 (humans) and Bai-hui (rats) increased functional connectivity between the anterior hypothalamus and the amygdala and mobilized mesenchymal stem cells (MSCs) into the systemic circulation. In human subjects, the source of the MSC was found to be primarily adipose tissue, whereas in rodents the tissue sources were considered more heterogeneous. Pharmacological disinhibition of rat hypothalamus enhanced sympathetic nervous system (SNS) activation and similarly resulted in a release of MSC into the circulation. EA-mediated SNS activation was further supported by browning of white adipose tissue in rats. EA treatment of rats undergoing partial rupture of the Achilles tendon resulted in reduced mechanical hyperalgesia, increased serum interleukin-10 levels and tendon remodeling, effects blocked in propranolol-treated rodents. To distinguish the afferent role of the peripheral nervous system, phosphoinositide-interacting regulator of transient receptor potential channels (Pirt)-GCaMP3 (genetically encoded calcium sensor) mice were treated with EA acupuncture points, ST-36 and LIV-3, and GV-14 and Bai-hui and resulted in a rapid activation of primary sensory neurons. EA activated sensory ganglia and SNS centers to mediate the release of MSC that can enhance tissue repair, increase anti-inflammatory cytokine production and provide pronounced analgesic relief. Stem Cells 2017;35:1303-1315.",

keywords = "Achilles Tendon/pathology, Acupuncture Points, Adipocytes/cytology, Adipose Tissue, Brown/cytology, Adipose Tissue, White/cytology, Animals, Antigens, CD/metabolism, Central Nervous System/cytology, Electroacupuncture, Forelimb/physiology, Hindlimb/physiology, Humans, Hyperalgesia/therapy, Hypothalamus/cytology, Interleukin-10/blood, Macrophages/cytology, Mesenchymal Stem Cells/cytology, Mice, Nerve Net/physiology, Rats, Rupture, Sensory Receptor Cells/metabolism, Uncoupling Protein 1/metabolism",

author = "Salazar, {Tatiana E} and Richardson, {Matthew R} and Eleni Beli and Ripsch, {Matthew S} and John George and Youngsook Kim and Yaqian Duan and Leni Moldovan and Yuanqing Yan and Ashay Bhatwadekar and Vaishnavi Jadhav and Smith, {Jared A} and Susan McGorray and Bertone, {Alicia L} and Traktuev, {Dmitri O} and March, {Keith L} and Colon-Perez, {Luis M} and Avin, {Keith G} and Emily Sims and Mund, {Julie A} and Jamie Case and Xiaolin Deng and Kim, {Min Su} and Bruce McDavitt and Boulton, {Michael E} and Jeffrey Thinschmidt and {Li Calzi}, Sergio and Fitz, {Stephanie D} and Fuchs, {Robyn K} and Warden, {Stuart J} and Todd McKinley and Anantha Shekhar and Marcelo Febo and Johnson, {Phillip L} and Lung-Ji Chang and Zhanguo Gao and Kolonin, {Mikhail G} and Song Lai and Jingfeng Ma and Xinzhong Dong and White, {Fletcher A} and Huisheng Xie and Yoder, {Mervin C} and Grant, {Maria B}",

note = "{\textcopyright} 2017 AlphaMed Press.",

year = "2017",

month = may,

doi = "10.1002/stem.2613",

language = "English",

volume = "35",

pages = "1303--1315",

journal = "Stem Cells",

issn = "1066-5099",

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Salazar, TE, Richardson, MR, Beli, E, Ripsch, MS, George, J, Kim, Y, Duan, Y, Moldovan, L, Yan, Y, Bhatwadekar, A, Jadhav, V, Smith, JA, McGorray, S, Bertone, AL, Traktuev, DO, March, KL, Colon-Perez, LM, Avin, KG, Sims, E, Mund, JA, Case, J, Deng, X, Kim, MS, McDavitt, B, Boulton, ME, Thinschmidt, J, Li Calzi, S, Fitz, SD, Fuchs, RK, Warden, SJ, McKinley, T, Shekhar, A, Febo, M, Johnson, PL, Chang, L-J, Gao, Z, Kolonin, MG, Lai, S, Ma, J, Dong, X, White, FA, Xie, H, Yoder, MC & Grant, MB 2017, 'Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells', Stem Cells, vol. 35, no. 5, pp. 1303-1315. https://doi.org/10.1002/stem.2613

TY - JOUR

T1 - Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells

AU - Salazar, Tatiana E

AU - Richardson, Matthew R

AU - Beli, Eleni

AU - Ripsch, Matthew S

AU - George, John

AU - Kim, Youngsook

AU - Duan, Yaqian

AU - Moldovan, Leni

AU - Yan, Yuanqing

AU - Bhatwadekar, Ashay

AU - Jadhav, Vaishnavi

AU - Smith, Jared A

AU - McGorray, Susan

AU - Bertone, Alicia L

AU - Traktuev, Dmitri O

AU - March, Keith L

AU - Colon-Perez, Luis M

AU - Avin, Keith G

AU - Sims, Emily

AU - Mund, Julie A

AU - Case, Jamie

AU - Deng, Xiaolin

AU - Kim, Min Su

AU - McDavitt, Bruce

AU - Boulton, Michael E

AU - Thinschmidt, Jeffrey

AU - Li Calzi, Sergio

AU - Fitz, Stephanie D

AU - Fuchs, Robyn K

AU - Warden, Stuart J

AU - McKinley, Todd

AU - Shekhar, Anantha

AU - Febo, Marcelo

AU - Johnson, Phillip L

AU - Chang, Lung-Ji

AU - Gao, Zhanguo

AU - Kolonin, Mikhail G

AU - Lai, Song

AU - Ma, Jingfeng

AU - Dong, Xinzhong

AU - White, Fletcher A

AU - Xie, Huisheng

AU - Yoder, Mervin C

AU - Grant, Maria B

PY - 2017/5

Y1 - 2017/5

N2 - Electroacupuncture (EA) performed in rats and humans using limb acupuncture sites, LI-4 and LI-11, and GV-14 and GV-20 (humans) and Bai-hui (rats) increased functional connectivity between the anterior hypothalamus and the amygdala and mobilized mesenchymal stem cells (MSCs) into the systemic circulation. In human subjects, the source of the MSC was found to be primarily adipose tissue, whereas in rodents the tissue sources were considered more heterogeneous. Pharmacological disinhibition of rat hypothalamus enhanced sympathetic nervous system (SNS) activation and similarly resulted in a release of MSC into the circulation. EA-mediated SNS activation was further supported by browning of white adipose tissue in rats. EA treatment of rats undergoing partial rupture of the Achilles tendon resulted in reduced mechanical hyperalgesia, increased serum interleukin-10 levels and tendon remodeling, effects blocked in propranolol-treated rodents. To distinguish the afferent role of the peripheral nervous system, phosphoinositide-interacting regulator of transient receptor potential channels (Pirt)-GCaMP3 (genetically encoded calcium sensor) mice were treated with EA acupuncture points, ST-36 and LIV-3, and GV-14 and Bai-hui and resulted in a rapid activation of primary sensory neurons. EA activated sensory ganglia and SNS centers to mediate the release of MSC that can enhance tissue repair, increase anti-inflammatory cytokine production and provide pronounced analgesic relief. Stem Cells 2017;35:1303-1315.

AB - Electroacupuncture (EA) performed in rats and humans using limb acupuncture sites, LI-4 and LI-11, and GV-14 and GV-20 (humans) and Bai-hui (rats) increased functional connectivity between the anterior hypothalamus and the amygdala and mobilized mesenchymal stem cells (MSCs) into the systemic circulation. In human subjects, the source of the MSC was found to be primarily adipose tissue, whereas in rodents the tissue sources were considered more heterogeneous. Pharmacological disinhibition of rat hypothalamus enhanced sympathetic nervous system (SNS) activation and similarly resulted in a release of MSC into the circulation. EA-mediated SNS activation was further supported by browning of white adipose tissue in rats. EA treatment of rats undergoing partial rupture of the Achilles tendon resulted in reduced mechanical hyperalgesia, increased serum interleukin-10 levels and tendon remodeling, effects blocked in propranolol-treated rodents. To distinguish the afferent role of the peripheral nervous system, phosphoinositide-interacting regulator of transient receptor potential channels (Pirt)-GCaMP3 (genetically encoded calcium sensor) mice were treated with EA acupuncture points, ST-36 and LIV-3, and GV-14 and Bai-hui and resulted in a rapid activation of primary sensory neurons. EA activated sensory ganglia and SNS centers to mediate the release of MSC that can enhance tissue repair, increase anti-inflammatory cytokine production and provide pronounced analgesic relief. Stem Cells 2017;35:1303-1315.

KW - Achilles Tendon/pathology

KW - Acupuncture Points

KW - Adipocytes/cytology

KW - Adipose Tissue, Brown/cytology

KW - Adipose Tissue, White/cytology

KW - Animals

KW - Antigens, CD/metabolism

KW - Central Nervous System/cytology

KW - Electroacupuncture

KW - Forelimb/physiology

KW - Hindlimb/physiology

KW - Humans

KW - Hyperalgesia/therapy

KW - Hypothalamus/cytology

KW - Interleukin-10/blood

KW - Macrophages/cytology

KW - Mesenchymal Stem Cells/cytology

KW - Mice

KW - Nerve Net/physiology

KW - Rats

KW - Rupture

KW - Sensory Receptor Cells/metabolism

KW - Uncoupling Protein 1/metabolism

U2 - 10.1002/stem.2613

DO - 10.1002/stem.2613

M3 - Article

C2 - 28299842

SN - 1066-5099

VL - 35

SP - 1303

EP - 1315

JO - Stem Cells

JF - Stem Cells

IS - 5

ER -

Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells

Abstract

Bibliographical note

Keywords

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