Electrochemical nanoprobe-based immunosensor for deoxynivalenol mycotoxin residues analysis in wheat samples

Enrique Valera*, Raül García-Febrero, Christopher T. Elliott, Francisco Sánchez-Baeza, M. P. Marco

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Deoxynivalenol (DON) is a toxic secondary metabolite produced by several species of Fusarium fungi, which can be predominantly found in agricultural crops such as wheat. In livestock, deoxynivalenol-contaminated grain can produce vomiting, feed refusal, weight loss, and diarrhea. This paper reports an electrochemical immunosensor for the detection of residual DON mycotoxin in food samples. The device uses electrochemical nanoprobes (CdSNP-AbDON) and antigen biofunctionalized magnetic μ-particles (DON-BSAMP) to detect the mycotoxin. CdSNP-AbDON are prepared by labelling the DON-specific antibodies with CdS nanoparticles (CdSNPs). Nanoparticle size and CdSNP-AbDON conjugation ratio are characterized using TEM images. The metal ions released by the CdSNP are reduced at the working electrode and read by anodic stripping voltammetry. DON can be detected in PBST buffer with an IC50 of 6.74 ± 0.19 μg L−1. The high detectability of the immunosensor developed allows detection of DON residues in 50-fold diluted wheat extracts. The limit of detection (LOD, IC90) accomplished in wheat is of 342.4 μg kg−1, which is below the maximum residue limit (MRL, 1750 μg kg−1 for unprocessed durum wheat, 750 μg kg−1 for cereals intended for direct human consumption) established by the EU for this mycotoxin. The working range is in the interval between 610 and 6210 μg kg−1. The performance of the immunosensor was compared with the ELISA assay. DON naturally contaminated wheat samples were analyzed with the immunosensor, showing acceptable recoveries.

Original languageEnglish
Pages (from-to)1915-1926
Number of pages12
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number9
Early online date04 Jan 2019
DOIs
Publication statusPublished - Mar 2019

Fingerprint

Nanoprobes
Immunosensors
Mycotoxins
Triticum
Nanoparticles
Agricultural Crops
deoxynivalenol
Poisons
Fusarium
Livestock
Voltammetry
Metabolites
Fungi
Farms
Labeling
Inhibitory Concentration 50
Crops
Vomiting
Metal ions
Limit of Detection

Keywords

  • CdS nanoparticles
  • Deoxynivalenol mycotoxin residues
  • Electrochemical nanoprobes
  • Food safety
  • Immunosensor
  • Wheat

Cite this

Valera, Enrique ; García-Febrero, Raül ; Elliott, Christopher T. ; Sánchez-Baeza, Francisco ; Marco, M. P. / Electrochemical nanoprobe-based immunosensor for deoxynivalenol mycotoxin residues analysis in wheat samples. In: Analytical and Bioanalytical Chemistry. 2019 ; Vol. 411, No. 9. pp. 1915-1926.
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abstract = "Deoxynivalenol (DON) is a toxic secondary metabolite produced by several species of Fusarium fungi, which can be predominantly found in agricultural crops such as wheat. In livestock, deoxynivalenol-contaminated grain can produce vomiting, feed refusal, weight loss, and diarrhea. This paper reports an electrochemical immunosensor for the detection of residual DON mycotoxin in food samples. The device uses electrochemical nanoprobes (CdSNP-AbDON) and antigen biofunctionalized magnetic μ-particles (DON-BSAMP) to detect the mycotoxin. CdSNP-AbDON are prepared by labelling the DON-specific antibodies with CdS nanoparticles (CdSNPs). Nanoparticle size and CdSNP-AbDON conjugation ratio are characterized using TEM images. The metal ions released by the CdSNP are reduced at the working electrode and read by anodic stripping voltammetry. DON can be detected in PBST buffer with an IC50 of 6.74 ± 0.19 μg L−1. The high detectability of the immunosensor developed allows detection of DON residues in 50-fold diluted wheat extracts. The limit of detection (LOD, IC90) accomplished in wheat is of 342.4 μg kg−1, which is below the maximum residue limit (MRL, 1750 μg kg−1 for unprocessed durum wheat, 750 μg kg−1 for cereals intended for direct human consumption) established by the EU for this mycotoxin. The working range is in the interval between 610 and 6210 μg kg−1. The performance of the immunosensor was compared with the ELISA assay. DON naturally contaminated wheat samples were analyzed with the immunosensor, showing acceptable recoveries.",
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Electrochemical nanoprobe-based immunosensor for deoxynivalenol mycotoxin residues analysis in wheat samples. / Valera, Enrique; García-Febrero, Raül; Elliott, Christopher T.; Sánchez-Baeza, Francisco; Marco, M. P.

In: Analytical and Bioanalytical Chemistry, Vol. 411, No. 9, 03.2019, p. 1915-1926.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Electrochemical nanoprobe-based immunosensor for deoxynivalenol mycotoxin residues analysis in wheat samples

AU - Valera, Enrique

AU - García-Febrero, Raül

AU - Elliott, Christopher T.

AU - Sánchez-Baeza, Francisco

AU - Marco, M. P.

PY - 2019/3

Y1 - 2019/3

N2 - Deoxynivalenol (DON) is a toxic secondary metabolite produced by several species of Fusarium fungi, which can be predominantly found in agricultural crops such as wheat. In livestock, deoxynivalenol-contaminated grain can produce vomiting, feed refusal, weight loss, and diarrhea. This paper reports an electrochemical immunosensor for the detection of residual DON mycotoxin in food samples. The device uses electrochemical nanoprobes (CdSNP-AbDON) and antigen biofunctionalized magnetic μ-particles (DON-BSAMP) to detect the mycotoxin. CdSNP-AbDON are prepared by labelling the DON-specific antibodies with CdS nanoparticles (CdSNPs). Nanoparticle size and CdSNP-AbDON conjugation ratio are characterized using TEM images. The metal ions released by the CdSNP are reduced at the working electrode and read by anodic stripping voltammetry. DON can be detected in PBST buffer with an IC50 of 6.74 ± 0.19 μg L−1. The high detectability of the immunosensor developed allows detection of DON residues in 50-fold diluted wheat extracts. The limit of detection (LOD, IC90) accomplished in wheat is of 342.4 μg kg−1, which is below the maximum residue limit (MRL, 1750 μg kg−1 for unprocessed durum wheat, 750 μg kg−1 for cereals intended for direct human consumption) established by the EU for this mycotoxin. The working range is in the interval between 610 and 6210 μg kg−1. The performance of the immunosensor was compared with the ELISA assay. DON naturally contaminated wheat samples were analyzed with the immunosensor, showing acceptable recoveries.

AB - Deoxynivalenol (DON) is a toxic secondary metabolite produced by several species of Fusarium fungi, which can be predominantly found in agricultural crops such as wheat. In livestock, deoxynivalenol-contaminated grain can produce vomiting, feed refusal, weight loss, and diarrhea. This paper reports an electrochemical immunosensor for the detection of residual DON mycotoxin in food samples. The device uses electrochemical nanoprobes (CdSNP-AbDON) and antigen biofunctionalized magnetic μ-particles (DON-BSAMP) to detect the mycotoxin. CdSNP-AbDON are prepared by labelling the DON-specific antibodies with CdS nanoparticles (CdSNPs). Nanoparticle size and CdSNP-AbDON conjugation ratio are characterized using TEM images. The metal ions released by the CdSNP are reduced at the working electrode and read by anodic stripping voltammetry. DON can be detected in PBST buffer with an IC50 of 6.74 ± 0.19 μg L−1. The high detectability of the immunosensor developed allows detection of DON residues in 50-fold diluted wheat extracts. The limit of detection (LOD, IC90) accomplished in wheat is of 342.4 μg kg−1, which is below the maximum residue limit (MRL, 1750 μg kg−1 for unprocessed durum wheat, 750 μg kg−1 for cereals intended for direct human consumption) established by the EU for this mycotoxin. The working range is in the interval between 610 and 6210 μg kg−1. The performance of the immunosensor was compared with the ELISA assay. DON naturally contaminated wheat samples were analyzed with the immunosensor, showing acceptable recoveries.

KW - CdS nanoparticles

KW - Deoxynivalenol mycotoxin residues

KW - Electrochemical nanoprobes

KW - Food safety

KW - Immunosensor

KW - Wheat

UR - http://www.mendeley.com/research/electrochemical-nanoprobebased-immunosensor-deoxynivalenol-mycotoxin-residues-analysis-wheat-samples

U2 - 10.1007/s00216-018-1538-0

DO - 10.1007/s00216-018-1538-0

M3 - Article

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VL - 411

SP - 1915

EP - 1926

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 9

ER -