Enhancement of cytochrome c catalytic behaviour by affecting the heme environment using functionalized carbon-based nanomaterials

Michaela Patila, Ioannis V. Pavlidis, Evmorfia K. Diamanti, Petros Katapodis, Dimitrios Gournis, Haralampos Stamatis

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

The effect of carbon-based nanomaterials (CBNs), such as multi-wall carbon nanotubes (CNTs) and graphene oxide (GO) nanomaterials functionalized with carboxyl, alkyl and amine groups, on the peroxidase-like activity and structure of cytochrome c (cyt c) was investigated. The catalytic efficiency of cyt c increases up to 78-fold in the presence of graphene oxide and up to 2.5-fold in the presence of other functionalized CBNs. Moreover, the use of functionalized CBNs enhances the thermal stability of the protein as well as its tolerance against hydrogen peroxide up to 2.5-fold. UV-vis and circular dichroism spectroscopy studies suggest that the increase in the peroxidase activity of cyt c in the presence of some functionalized GO nanomaterials, correlates to perturbations of the heme microenvironment, while the secondary structure of the enzyme remains intact. These results indicate that the beneficial effect the functionalized CBNs have on the activity and on the stability of cyt c depends on CBNs geometry and surface functionalization. ?? 2013 Elsevier Ltd.
Original languageEnglish
Pages (from-to)1010-1017
Number of pages8
JournalProcess Biochemistry
Volume48
Issue number7
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Carbon nanotubes
  • Carbon-based nanomaterials
  • Cytochrome c
  • Graphene oxide
  • Peroxidase activity

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