Enhancement of NMDA responses by β-amyloid peptides in the hippocampus in vivo

Zsolt Molnár; Katalin Soós; Imre Lengyel; Botond Penke; Viktor Szegedi; Dénes Budai

doi:10.1097/01.wnr.0000134471.06244.d2

Enhancement of NMDA responses by β-amyloid peptides in the hippocampus in vivo

Zsolt Molnár, Katalin Soós, Imre Lengyel, Botond Penke, Viktor Szegedi, Dénes Budai^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

The effects of Alzheimer's disease-related beta-amyloid (Aβ) peptides on the N-methyl-D-aspartate (NMDA)-evoked cell firing rate were studied in hippocampal CA1 neurons of the rat. Extracellular single-unit recordings were combined with iontophoretic applications that allowed quantitative analyses of the interactions between Aβ peptides and NMDA receptor-mediated events in vivo. The NMDA responses were significantly increased both by the full length Aβ1-42 and by its model fragment Aβ25-35. Enhancements of the NMDA responses by the Aβ peptides lasted about 15 min and were irreversible. The effects of Aβ25-35 were prevented by the pentapeptide Lys-Leu-Val-Gly-Phe- amide (KLVGF) and were not evoked when its reversed sequence (Aβ35-25) was applied.

Original language	English
Pages (from-to)	1649-1652
Number of pages	4
Journal	Neuroreport
Volume	15
Issue number	10
DOIs	https://doi.org/10.1097/01.wnr.0000134471.06244.d2
Publication status	Published - 19 Jul 2004
Externally published	Yes

Keywords

Alzheimer's disease
Amyloid β-peptide
Amyloid fragment
Microiontophoresis
NMDA
Rat
Single-unit recording

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1097/01.wnr.0000134471.06244.d2

Cite this

@article{f7f11d694a384e2fa1c550444da7bf8f,

title = "Enhancement of NMDA responses by β-amyloid peptides in the hippocampus in vivo",

abstract = "The effects of Alzheimer's disease-related beta-amyloid (Aβ) peptides on the N-methyl-D-aspartate (NMDA)-evoked cell firing rate were studied in hippocampal CA1 neurons of the rat. Extracellular single-unit recordings were combined with iontophoretic applications that allowed quantitative analyses of the interactions between Aβ peptides and NMDA receptor-mediated events in vivo. The NMDA responses were significantly increased both by the full length Aβ1-42 and by its model fragment Aβ25-35. Enhancements of the NMDA responses by the Aβ peptides lasted about 15 min and were irreversible. The effects of Aβ25-35 were prevented by the pentapeptide Lys-Leu-Val-Gly-Phe- amide (KLVGF) and were not evoked when its reversed sequence (Aβ35-25) was applied.",

keywords = "Alzheimer's disease, Amyloid β-peptide, Amyloid fragment, Microiontophoresis, NMDA, Rat, Single-unit recording",

author = "Zsolt Moln{\'a}r and Katalin So{\'o}s and Imre Lengyel and Botond Penke and Viktor Szegedi and D{\'e}nes Budai",

year = "2004",

month = jul,

day = "19",

doi = "10.1097/01.wnr.0000134471.06244.d2",

language = "English",

volume = "15",

pages = "1649--1652",

journal = "Neuroreport",

issn = "0959-4965",

publisher = "Lippincott Williams and Wilkins",

number = "10",

}

TY - JOUR

T1 - Enhancement of NMDA responses by β-amyloid peptides in the hippocampus in vivo

AU - Molnár, Zsolt

AU - Soós, Katalin

AU - Lengyel, Imre

AU - Penke, Botond

AU - Szegedi, Viktor

AU - Budai, Dénes

PY - 2004/7/19

Y1 - 2004/7/19

N2 - The effects of Alzheimer's disease-related beta-amyloid (Aβ) peptides on the N-methyl-D-aspartate (NMDA)-evoked cell firing rate were studied in hippocampal CA1 neurons of the rat. Extracellular single-unit recordings were combined with iontophoretic applications that allowed quantitative analyses of the interactions between Aβ peptides and NMDA receptor-mediated events in vivo. The NMDA responses were significantly increased both by the full length Aβ1-42 and by its model fragment Aβ25-35. Enhancements of the NMDA responses by the Aβ peptides lasted about 15 min and were irreversible. The effects of Aβ25-35 were prevented by the pentapeptide Lys-Leu-Val-Gly-Phe- amide (KLVGF) and were not evoked when its reversed sequence (Aβ35-25) was applied.

AB - The effects of Alzheimer's disease-related beta-amyloid (Aβ) peptides on the N-methyl-D-aspartate (NMDA)-evoked cell firing rate were studied in hippocampal CA1 neurons of the rat. Extracellular single-unit recordings were combined with iontophoretic applications that allowed quantitative analyses of the interactions between Aβ peptides and NMDA receptor-mediated events in vivo. The NMDA responses were significantly increased both by the full length Aβ1-42 and by its model fragment Aβ25-35. Enhancements of the NMDA responses by the Aβ peptides lasted about 15 min and were irreversible. The effects of Aβ25-35 were prevented by the pentapeptide Lys-Leu-Val-Gly-Phe- amide (KLVGF) and were not evoked when its reversed sequence (Aβ35-25) was applied.

KW - Alzheimer's disease

KW - Amyloid β-peptide

KW - Amyloid fragment

KW - Microiontophoresis

KW - NMDA

KW - Rat

KW - Single-unit recording

UR - http://www.scopus.com/inward/record.url?scp=3242786300&partnerID=8YFLogxK

U2 - 10.1097/01.wnr.0000134471.06244.d2

DO - 10.1097/01.wnr.0000134471.06244.d2

M3 - Article

C2 - 15232300

AN - SCOPUS:3242786300

SN - 0959-4965

VL - 15

SP - 1649

EP - 1652

JO - Neuroreport

JF - Neuroreport

IS - 10

ER -

Enhancement of NMDA responses by β-amyloid peptides in the hippocampus in vivo

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this