Entrapment in HydrIL gels: hydro-ionic liquid polymer gels for enzyme immobilization

José Ángel Pérez-Tomás, Rebekah Brucato, Preston Griffin, Jakub Kostal, Gareth Brown, Stefan Mix, Patricia C. Marr*, Andrew C. Marr*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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Abstract

Enzyme entrapment in hydro-ionic liquid gels (HydrIL gels) was explored as an enzyme immobilization strategy. The liquid phase of the gels was a mixture of buffer and tributyl methyl phosphonium alkanesulfonate [P4441][RSO3] ionic liquids. The ionic liquid modifies the liquid phase creating a more “organic” hydrophobic microenvironment around the enzyme. In the esterification of a secondary alcohol by Candida antarctica Lipase B, a 20-fold enhancement in final substrate conversion was achieved using an HydrIL gel compared to a standard poly(acrylamide) hydrogel. Co-polymerization of the poly(acrylamide) matrix with acrylate monomers led to further increases in reaction rate. A CalB HydrIL gel was reused over 10 reaction cycles, achieving a consistent performance from the fifth cycle. IL leaching became undetectable from the third reaction cycle.

Original languageEnglish
Article number 114595
Number of pages12
JournalCatalysis Today
Volume432
Early online date23 Feb 2024
DOIs
Publication statusPublished - 15 Apr 2024

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