Epidemiological study on prevalence, serovar diversity, multidrug resistance, and CTX-M-type extended-spectrum β-lactamases of salmonella spp. From patients with diarrhea, food of animal origin, and pets in Several Provinces of China

Wei Wang, Li Zhao, Yujie Hu, Tania Dottorini, Séamus Fanning, Jin Xu*, Fengqin Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

A total of 2,283 Salmonella isolates were recovered from 18,334 samples, including samples from patients with diarrhea, food of animal origin, and pets, across 5 provinces of China. The highest prevalence of Salmonella spp. was detected in chicken meats (39.3%, 486/1,237). Fifteen serogroups and 66 serovars were identified, with Salmonella enterica serovars Typhimurium and Enteritidis being the most dominant. Most (85.5%, 1,952/2,283) isolates exhibited resistance to ≥1 antimicrobial, and 56.4% were multidrug resistant (MDR). A total of 222 isolates harbored extended-spectrum β-lactamases (ESBLs), and 200 of these were of the CTX-Mtype and were mostly detected in isolates from chicken meat and turtle fecal samples. Overall, eight blaCTX-Mgenes were identified, with blaCTX-M-65, blaCTX-M-123, blaCTX-M-14, blaCTX-M-79, and blaCTX-M-130being the most prevalent. In total, 166 of the 222 ESBLproducing isolates had amino acid substitutions in GyrA (S83Y, S83F, D87G, D87N, and D87Y) and ParC (S80I), while the plasmid-mediated quinolone resistance (PMQR)-encoding genes oqxA, oqxB, qepA, qnrB, and qnrS were detected in almost all isolates. Of the 15 sequence types (STs) identified in the 222 ESBLs, ST17, ST11, ST34, and ST26 ranked among the top 5 in number of isolates. Our study revealed considerable serovar diversity and a high prevalence of the co-occurrence of MDR determinants, including CTX-M-type ESBLs, quinolone resistance-determining region (QRDR) mutations, and PMQR genes. This is the first report of CTX-M-130 Salmonella spp. from patients with diarrhea and QRDR mutations from turtle fecal samples. Our study emphasizes the importance of actions, both in health care settings and in the veterinary medicine sector, to control the dissemination of MDR, especially the CTXM- type ESBL-harboring Salmonella isolates.

Original languageEnglish
Article numbere00092-20
JournalAntimicrobial Agents and Chemotherapy
Volume64
Issue number7
Early online date20 Apr 2020
DOIs
Publication statusPublished - 23 Jun 2020

Bibliographical note

Funding Information:
This study was funded by the National Key R&D Program of China (grant 2016YFD0401102) and China Food Safety Talent Competency Development Initiative: CFSA 523 Program.

Publisher Copyright:
© 2020 American Society for Microbiology. All rights reserved.

Keywords

  • Antimicrobial resistance
  • CTX-M
  • MLST
  • PMQR
  • QRDRs
  • Salmonella spp.

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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