Erythropoietin production in kidney tubular cells

A P Maxwell, T R Lappin, C F Johnston, J M Bridges, M G McGeown

Research output: Contribution to journalArticlepeer-review

89 Citations (Scopus)

Abstract

The erythropoietin gene has been cloned in three mammalian species including man and recombinant erythropoietin is now used to treat the anaemia of chronic renal failure. Despite the isolation of the gene the precise cellular location of erythropoietin synthesis remains controversial. We present studies which demonstrate erythropoietin production by kidney tubular cells. Erythropoietin gene expression (messenger RNA) was detected by in situ hybridization using an oligonucleotide gene probe and the translated protein product by immunohistochemistry employing antibodies raised to pure recombinant DNA derived erythropoietin.
Original languageEnglish
Pages (from-to)535-9
Number of pages5
JournalBritish Journal of Haematology
Volume74
Issue number4
Publication statusPublished - 1990

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