Estimation of the surface area and volume of the retinal capillary basement membrane using the stereologic method of vertical sections

Heather Anderson, Alan Stitt, T. A. Gardiner, D. B. Archer

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The modern stereologic method of vertical sections was applied to the retina as a means of generating unbiased estimates of three-dimensional structure. The method is illustrated with real data on the volume and surface area of the capillary basement membrane from the central retina of the rat. Novel methods of estimating the volume of retina sampled and of creating accurate vertical sections are described. The advantages of using stereologic methods to generate quantitative information on the three-dimensional structure of the retina are discussed and compared to those of previous quantitative methods that provide data on two-dimensional structure only.
Original languageEnglish
Pages (from-to)253-260
Number of pages8
JournalAnalytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology
Volume16
Issue number4
Publication statusPublished - 01 Aug 1994

Bibliographical note

LR: 20061115; JID: 8506819; ppublish

Keywords

  • Animals
  • Basement Membrane/ultrastructure
  • Capillaries/ultrastructure
  • Image Processing, Computer-Assisted/methods
  • Male
  • Microscopy, Electron/methods
  • Microtomy/methods
  • Rats
  • Rats, Wistar
  • Retina/ultrastructure

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