Estimation of the surface area and volume of the retinal capillary basement membrane using the stereologic method of vertical sections

Heather Anderson; Alan Stitt; T. A. Gardiner; D. B. Archer

Estimation of the surface area and volume of the retinal capillary basement membrane using the stereologic method of vertical sections

Heather Anderson, Alan Stitt, T. A. Gardiner, D. B. Archer

Research output: Contribution to journal › Article › peer-review

Abstract

The modern stereologic method of vertical sections was applied to the retina as a means of generating unbiased estimates of three-dimensional structure. The method is illustrated with real data on the volume and surface area of the capillary basement membrane from the central retina of the rat. Novel methods of estimating the volume of retina sampled and of creating accurate vertical sections are described. The advantages of using stereologic methods to generate quantitative information on the three-dimensional structure of the retina are discussed and compared to those of previous quantitative methods that provide data on two-dimensional structure only.

Original language	English
Pages (from-to)	253-260
Number of pages	8
Journal	Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology
Volume	16
Issue number	4
Publication status	Published - 01 Aug 1994

Bibliographical note

LR: 20061115; JID: 8506819; ppublish

Keywords

Animals
Basement Membrane/ultrastructure
Capillaries/ultrastructure
Image Processing, Computer-Assisted/methods
Male
Microscopy, Electron/methods
Microtomy/methods
Rats
Rats, Wistar
Retina/ultrastructure

Cite this

Anderson, Heather ; Stitt, Alan ; Gardiner, T. A. ; Archer, D. B. / Estimation of the surface area and volume of the retinal capillary basement membrane using the stereologic method of vertical sections. In: Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology. 1994 ; Vol. 16, No. 4. pp. 253-260.

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title = "Estimation of the surface area and volume of the retinal capillary basement membrane using the stereologic method of vertical sections",

abstract = "The modern stereologic method of vertical sections was applied to the retina as a means of generating unbiased estimates of three-dimensional structure. The method is illustrated with real data on the volume and surface area of the capillary basement membrane from the central retina of the rat. Novel methods of estimating the volume of retina sampled and of creating accurate vertical sections are described. The advantages of using stereologic methods to generate quantitative information on the three-dimensional structure of the retina are discussed and compared to those of previous quantitative methods that provide data on two-dimensional structure only.",

keywords = "Animals, Basement Membrane/ultrastructure, Capillaries/ultrastructure, Image Processing, Computer-Assisted/methods, Male, Microscopy, Electron/methods, Microtomy/methods, Rats, Rats, Wistar, Retina/ultrastructure",

author = "Heather Anderson and Alan Stitt and Gardiner, {T. A.} and Archer, {D. B.}",

note = "LR: 20061115; JID: 8506819; ppublish",

year = "1994",

month = aug,

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language = "English",

volume = "16",

pages = "253--260",

journal = "Analytical and quantitative cytology and histology",

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Estimation of the surface area and volume of the retinal capillary basement membrane using the stereologic method of vertical sections. / Anderson, Heather; Stitt, Alan; Gardiner, T. A.; Archer, D. B.

In: Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology, Vol. 16, No. 4, 01.08.1994, p. 253-260.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Estimation of the surface area and volume of the retinal capillary basement membrane using the stereologic method of vertical sections

AU - Anderson, Heather

AU - Stitt, Alan

AU - Gardiner, T. A.

AU - Archer, D. B.

N1 - LR: 20061115; JID: 8506819; ppublish

PY - 1994/8/1

Y1 - 1994/8/1

N2 - The modern stereologic method of vertical sections was applied to the retina as a means of generating unbiased estimates of three-dimensional structure. The method is illustrated with real data on the volume and surface area of the capillary basement membrane from the central retina of the rat. Novel methods of estimating the volume of retina sampled and of creating accurate vertical sections are described. The advantages of using stereologic methods to generate quantitative information on the three-dimensional structure of the retina are discussed and compared to those of previous quantitative methods that provide data on two-dimensional structure only.

AB - The modern stereologic method of vertical sections was applied to the retina as a means of generating unbiased estimates of three-dimensional structure. The method is illustrated with real data on the volume and surface area of the capillary basement membrane from the central retina of the rat. Novel methods of estimating the volume of retina sampled and of creating accurate vertical sections are described. The advantages of using stereologic methods to generate quantitative information on the three-dimensional structure of the retina are discussed and compared to those of previous quantitative methods that provide data on two-dimensional structure only.

KW - Animals

KW - Basement Membrane/ultrastructure

KW - Capillaries/ultrastructure

KW - Image Processing, Computer-Assisted/methods

KW - Male

KW - Microscopy, Electron/methods

KW - Microtomy/methods

KW - Rats

KW - Rats, Wistar

KW - Retina/ultrastructure

M3 - Article

C2 - 7945701

VL - 16

SP - 253

EP - 260

JO - Analytical and quantitative cytology and histology

JF - Analytical and quantitative cytology and histology

SN - 0884-6812

IS - 4

ER -

Estimation of the surface area and volume of the retinal capillary basement membrane using the stereologic method of vertical sections

Abstract

Bibliographical note

Keywords

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Cite this