EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations.

A.W. Langerak; P.J. Groenen; M. Brüggemann; K. Beldjord; C. Bellan; L. Bonello; E. Boone; G.I. Carter; M. Catherwood; F. Davi; M.H. Delfau-Larue; T. Diss; P.A. Evans; P. Gameiro; R. Garcia Sanz; D. Gonzalez; D. Grand; A. Håkansson; M. Hummel; H. Liu; L. Lombardia; E.A. Macintyre; B.J. Milner; S. Montes-Moreno; E. Schuuring; M. Spaargaren; E. Hodges; J.J. van Dongen

doi:10.1038/leu.2012.246

EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations.

A.W. Langerak, P.J. Groenen, M. Brüggemann, K. Beldjord, C. Bellan, L. Bonello, E. Boone, G.I. Carter, M. Catherwood, F. Davi, M.H. Delfau-Larue, T. Diss, P.A. Evans, P. Gameiro, R. Garcia Sanz, D. Gonzalez, D. Grand, A. Håkansson, M. Hummel, H. LiuL. Lombardia, E.A. Macintyre, B.J. Milner, S. Montes-Moreno, E. Schuuring, M. Spaargaren, E. Hodges, J.J. van Dongen

School of Medicine, Dentistry and Biomedical Sciences

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264 Citations (Scopus)

Abstract

PCR-based immunoglobulin (Ig)/T-cell receptor (TCR) clonality testing in suspected lymphoproliferations has largely been standardized and has consequently become technically feasible in a routine diagnostic setting. Standardization of the pre-analytical and post-analytical phases is now essential to prevent misinterpretation and incorrect conclusions derived from clonality data. As clonality testing is not a quantitative assay, but rather concerns recognition of molecular patterns, guidelines for reliable interpretation and reporting are mandatory. Here, the EuroClonality (BIOMED-2) consortium summarizes important pre- and post-analytical aspects of clonality testing, provides guidelines for interpretation of clonality testing results, and presents a uniform way to report the results of the Ig/TCR assays. Starting from an immunobiological concept, two levels to report Ig/TCR profiles are discerned: the technical description of individual (multiplex) PCR reactions and the overall molecular conclusion for B and T cells. Collectively, the EuroClonality (BIOMED-2) guidelines and consensus reporting system should help to improve the general performance level of clonality assessment and interpretation, which will directly impact on routine clinical management (standardized best-practice) in patients with suspected lymphoproliferations.

Original language	English
Pages (from-to)	2159-2171
Number of pages	13
Journal	Leukemia
Volume	26
Issue number	10
DOIs	https://doi.org/10.1038/leu.2012.246
Publication status	Published - Oct 2012

Keywords

DNA
Gene Rearrangement
Guidelines as Topic
Humans
Immunoglobulins
Lymphoproliferative Disorders
Multiplex Polymerase Chain Reaction
Receptors, Antigen, T-Cell

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10.1038/leu.2012.246

http://www.ncbi.nlm.nih.gov/pubmed/22918122

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Langerak, A. W., Groenen, P. J., Brüggemann, M., Beldjord, K., Bellan, C., Bonello, L., Boone, E., Carter, G. I., Catherwood, M., Davi, F., Delfau-Larue, M. H., Diss, T., Evans, P. A., Gameiro, P., Garcia Sanz, R., Gonzalez, D., Grand, D., Håkansson, A., Hummel, M., ... van Dongen, J. J. (2012). EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations. Leukemia, 26(10), 2159-2171. https://doi.org/10.1038/leu.2012.246

Langerak, A.W. ; Groenen, P.J. ; Brüggemann, M. ; Beldjord, K. ; Bellan, C. ; Bonello, L. ; Boone, E. ; Carter, G.I. ; Catherwood, M. ; Davi, F. ; Delfau-Larue, M.H. ; Diss, T. ; Evans, P.A. ; Gameiro, P. ; Garcia Sanz, R. ; Gonzalez, D. ; Grand, D. ; Håkansson, A. ; Hummel, M. ; Liu, H. ; Lombardia, L. ; Macintyre, E.A. ; Milner, B.J. ; Montes-Moreno, S. ; Schuuring, E. ; Spaargaren, M. ; Hodges, E. ; van Dongen, J.J. / EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations. In: Leukemia. 2012 ; Vol. 26, No. 10. pp. 2159-2171.

@article{33c0b7690f0b4cb7bbab488df7b34b3a,

title = "EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations.",

abstract = "PCR-based immunoglobulin (Ig)/T-cell receptor (TCR) clonality testing in suspected lymphoproliferations has largely been standardized and has consequently become technically feasible in a routine diagnostic setting. Standardization of the pre-analytical and post-analytical phases is now essential to prevent misinterpretation and incorrect conclusions derived from clonality data. As clonality testing is not a quantitative assay, but rather concerns recognition of molecular patterns, guidelines for reliable interpretation and reporting are mandatory. Here, the EuroClonality (BIOMED-2) consortium summarizes important pre- and post-analytical aspects of clonality testing, provides guidelines for interpretation of clonality testing results, and presents a uniform way to report the results of the Ig/TCR assays. Starting from an immunobiological concept, two levels to report Ig/TCR profiles are discerned: the technical description of individual (multiplex) PCR reactions and the overall molecular conclusion for B and T cells. Collectively, the EuroClonality (BIOMED-2) guidelines and consensus reporting system should help to improve the general performance level of clonality assessment and interpretation, which will directly impact on routine clinical management (standardized best-practice) in patients with suspected lymphoproliferations.",

keywords = "DNA, Gene Rearrangement, Guidelines as Topic, Humans, Immunoglobulins, Lymphoproliferative Disorders, Multiplex Polymerase Chain Reaction, Receptors, Antigen, T-Cell",

author = "A.W. Langerak and P.J. Groenen and M. Br{\"u}ggemann and K. Beldjord and C. Bellan and L. Bonello and E. Boone and G.I. Carter and M. Catherwood and F. Davi and M.H. Delfau-Larue and T. Diss and P.A. Evans and P. Gameiro and {Garcia Sanz}, R. and D. Gonzalez and D. Grand and A. H{\aa}kansson and M. Hummel and H. Liu and L. Lombardia and E.A. Macintyre and B.J. Milner and S. Montes-Moreno and E. Schuuring and M. Spaargaren and E. Hodges and {van Dongen}, J.J.",

year = "2012",

month = oct,

doi = "10.1038/leu.2012.246",

language = "English",

volume = "26",

pages = "2159--2171",

journal = "Leukemia",

issn = "0887-6924",

publisher = "NATURE PUBLISHING GROUP",

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Langerak, AW, Groenen, PJ, Brüggemann, M, Beldjord, K, Bellan, C, Bonello, L, Boone, E, Carter, GI, Catherwood, M, Davi, F, Delfau-Larue, MH, Diss, T, Evans, PA, Gameiro, P, Garcia Sanz, R, Gonzalez, D, Grand, D, Håkansson, A, Hummel, M, Liu, H, Lombardia, L, Macintyre, EA, Milner, BJ, Montes-Moreno, S, Schuuring, E, Spaargaren, M, Hodges, E & van Dongen, JJ 2012, 'EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations.', Leukemia, vol. 26, no. 10, pp. 2159-2171. https://doi.org/10.1038/leu.2012.246

EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations. / Langerak, A.W.; Groenen, P.J.; Brüggemann, M.; Beldjord, K.; Bellan, C.; Bonello, L.; Boone, E.; Carter, G.I.; Catherwood, M.; Davi, F.; Delfau-Larue, M.H.; Diss, T.; Evans, P.A.; Gameiro, P.; Garcia Sanz, R.; Gonzalez, D.; Grand, D.; Håkansson, A.; Hummel, M.; Liu, H.; Lombardia, L.; Macintyre, E.A.; Milner, B.J.; Montes-Moreno, S.; Schuuring, E.; Spaargaren, M.; Hodges, E.; van Dongen, J.J.

In: Leukemia, Vol. 26, No. 10, 10.2012, p. 2159-2171.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations.

AU - Langerak, A.W.

AU - Groenen, P.J.

AU - Brüggemann, M.

AU - Beldjord, K.

AU - Bellan, C.

AU - Bonello, L.

AU - Boone, E.

AU - Carter, G.I.

AU - Catherwood, M.

AU - Davi, F.

AU - Delfau-Larue, M.H.

AU - Diss, T.

AU - Evans, P.A.

AU - Gameiro, P.

AU - Garcia Sanz, R.

AU - Gonzalez, D.

AU - Grand, D.

AU - Håkansson, A.

AU - Hummel, M.

AU - Liu, H.

AU - Lombardia, L.

AU - Macintyre, E.A.

AU - Milner, B.J.

AU - Montes-Moreno, S.

AU - Schuuring, E.

AU - Spaargaren, M.

AU - Hodges, E.

AU - van Dongen, J.J.

PY - 2012/10

Y1 - 2012/10

N2 - PCR-based immunoglobulin (Ig)/T-cell receptor (TCR) clonality testing in suspected lymphoproliferations has largely been standardized and has consequently become technically feasible in a routine diagnostic setting. Standardization of the pre-analytical and post-analytical phases is now essential to prevent misinterpretation and incorrect conclusions derived from clonality data. As clonality testing is not a quantitative assay, but rather concerns recognition of molecular patterns, guidelines for reliable interpretation and reporting are mandatory. Here, the EuroClonality (BIOMED-2) consortium summarizes important pre- and post-analytical aspects of clonality testing, provides guidelines for interpretation of clonality testing results, and presents a uniform way to report the results of the Ig/TCR assays. Starting from an immunobiological concept, two levels to report Ig/TCR profiles are discerned: the technical description of individual (multiplex) PCR reactions and the overall molecular conclusion for B and T cells. Collectively, the EuroClonality (BIOMED-2) guidelines and consensus reporting system should help to improve the general performance level of clonality assessment and interpretation, which will directly impact on routine clinical management (standardized best-practice) in patients with suspected lymphoproliferations.

AB - PCR-based immunoglobulin (Ig)/T-cell receptor (TCR) clonality testing in suspected lymphoproliferations has largely been standardized and has consequently become technically feasible in a routine diagnostic setting. Standardization of the pre-analytical and post-analytical phases is now essential to prevent misinterpretation and incorrect conclusions derived from clonality data. As clonality testing is not a quantitative assay, but rather concerns recognition of molecular patterns, guidelines for reliable interpretation and reporting are mandatory. Here, the EuroClonality (BIOMED-2) consortium summarizes important pre- and post-analytical aspects of clonality testing, provides guidelines for interpretation of clonality testing results, and presents a uniform way to report the results of the Ig/TCR assays. Starting from an immunobiological concept, two levels to report Ig/TCR profiles are discerned: the technical description of individual (multiplex) PCR reactions and the overall molecular conclusion for B and T cells. Collectively, the EuroClonality (BIOMED-2) guidelines and consensus reporting system should help to improve the general performance level of clonality assessment and interpretation, which will directly impact on routine clinical management (standardized best-practice) in patients with suspected lymphoproliferations.

KW - DNA

KW - Gene Rearrangement

KW - Guidelines as Topic

KW - Humans

KW - Immunoglobulins

KW - Lymphoproliferative Disorders

KW - Multiplex Polymerase Chain Reaction

KW - Receptors, Antigen, T-Cell

U2 - 10.1038/leu.2012.246

DO - 10.1038/leu.2012.246

M3 - Article

VL - 26

SP - 2159

EP - 2171

JO - Leukemia

JF - Leukemia

SN - 0887-6924

IS - 10

ER -

EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations.

Abstract

Keywords

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