Evaluating virtual staining for high-throughput screening

Samuel Tonks, Chih-Yang Hsu, Steve Hood, Ryan Musso, Ceriden Hopely, Steve Titus, Alexander Krull, Minh Doan, Iain Styles

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)
11 Downloads (Pure)


Little is known about the feasibility of virtual staining for industry applications such as high-throughput screening (HTS). We provide a thorough analysis of the usability of image-to-image translation for the virtual staining of label-free bright-field microscopy images of live cells, using a pool of more than 1.6 million images across six lung, six ovarian and six breast cell lines consisting of paired bright-field, cytoplasm, nuclei and DNA-damage stains. To our knowledge this is the first time an analysis of virtual staining has been performed on three levels; pixel-based, biological-feature based, and determining if virtual staining can reproduce drug-effect. Our results reveal that while virtually stained nuclei and cytoplasm images often consistently and faithfully reproduce the information found in fluorescence microscopy, virtually stained images of DNA-damage are usually less accurate.

Original languageEnglish
Title of host publicationProceedings of the 20th IEEE International Symposium on Biomedical Imaging, ISBI 2023
PublisherInstitute of Electrical and Electronics Engineers Inc.
Number of pages5
ISBN (Electronic)9781665473583
ISBN (Print)9781665473590
Publication statusPublished - 01 Sept 2023
Externally publishedYes
Event20th IEEE International Symposium on Biomedical Imaging 2023 - Cartagena de Indias, Colombia
Duration: 18 Apr 202321 Apr 2023

Publication series

NameIEEE International Symposium on Biomedical Imaging: proceedings
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452


Conference20th IEEE International Symposium on Biomedical Imaging 2023
Abbreviated titleISBI 20203
CityCartagena de Indias
Internet address


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