Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research

Grace C. Roberts; Carsten Zothner; Roland Remenyi; Andres Merits; Nicola J. Stonehouse; Mark Harris

doi:10.1038/s41598-017-15269-w

Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research

Grace C. Roberts, Carsten Zothner, Roland Remenyi, Andres Merits, Nicola J. Stonehouse, Mark Harris^*

^*Corresponding author for this work

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

Chikungunya virus (CHIKV) is becoming an increasing global health issue which has spread across the globe and as far north as southern Europe. There is currently no vaccine or anti-viral treatment available. Although there has been a recent increase in CHIKV research, many of these in vitro studies have used a wide range of cell lines which are not physiologically relevant to CHIKV infection in vivo. In this study, we aimed to evaluate a panel of cell lines to identify a subset that would be both representative of the infectious cycle of CHIKV in vivo, and amenable to in vitro applications such as transfection, luciferase assays, immunofluorescence, western blotting and virus infection. Based on these parameters we selected four mammalian and two mosquito cell lines, and further characterised these as potential tools in CHIKV research.

Original language	English
Article number	14641
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-15269-w
Publication status	Published - 01 Dec 2017

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Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research
Copyright 2017 Elsevier. This manuscript is distributed under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.
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Cite this

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AU - Stonehouse, Nicola J.

AU - Harris, Mark

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Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research

Abstract

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