Abstract
Glycated insulin was evaluated in plasma and biological tissues of diabetic animal models by immuno. cytochemistry (ICC) and a novel radioimmunoassay. Glycated insulin circulated at 0.10 +/-0.04 ng/ml and 2.20 +/-0.14 ng/ml in lean and diabetic obese (ob/ob) mice, corresponding to 12.5 and 9.8% total plasma insulin, respectively. The concentration of glycated insulin was elevated 22-fold in obese mice compared to controls (P10 and 83 +/-4 ng/g wt (P0.17 mug/g wt). ICC revealed fluorescent positively stained cells in pancreatic islets from hydrocortisone (HC)treated diabetic rats. Fasting of HC-treated rats, resulted in 3-fold and 15-fold reductions in plasma glycated insulin (P
| Original language | English |
|---|---|
| Pages (from-to) | 524-528 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 286 |
| Issue number | 3 |
| Publication status | Published - 2001 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
