Evaluation of glycated insulin in diabetic animals using immunocytochemistry and radioimmunoassay

  • A.M. McKillop
  • , Mark Mooney
  • , Patrick Harriott
  • , P.R. Flatt
  • , F.P.M. O'Harte

Research output: Contribution to journalArticlepeer-review

Abstract

Glycated insulin was evaluated in plasma and biological tissues of diabetic animal models by immuno. cytochemistry (ICC) and a novel radioimmunoassay. Glycated insulin circulated at 0.10 +/-0.04 ng/ml and 2.20 +/-0.14 ng/ml in lean and diabetic obese (ob/ob) mice, corresponding to 12.5 and 9.8% total plasma insulin, respectively. The concentration of glycated insulin was elevated 22-fold in obese mice compared to controls (P10 and 83 +/-4 ng/g wt (P0.17 mug/g wt). ICC revealed fluorescent positively stained cells in pancreatic islets from hydrocortisone (HC)treated diabetic rats. Fasting of HC-treated rats, resulted in 3-fold and 15-fold reductions in plasma glycated insulin (P
Original languageEnglish
Pages (from-to)524-528
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume286
Issue number3
Publication statusPublished - 2001

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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