Evaluation of HER-2/neu oncogene status in breast tumors on tissue microarrays

D.H. Zhang, Manuel Salto-Tellez, E. Do, T.C. Putti, E.S.C. Koay

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)


The amplification and/or overexpression of the HER-2/neu oncogene and its encoded receptor protein are increasingly used for prognostication and prediction of therapeutic response to Herceptin in breast cancer. However, large-scale examination of archival tumor blocks by immunohistochemistry (IHC) or fluorescence in situ hybridization (FISH) is prohibitively laborious and technically challenging. The tissue microarray (TMA) technique enables hundreds of tumors to be studied simultaneously in a single experiment. To evaluate the HER-2/neu status of a selection of the breast tumors in our tumor bank, we constructed a TMA from 97 breast tumors, with a single 0.6-mm core per specimen. HER-2/neu gene amplification by FISH was found in 20 of the 87 interpretable cases (23%): in 14 of 14 IHC 3+ cases (100%), 5 of 8 IHC 2+ cases (62.5%) and 1 of 65 IHC 0/1+ cases (1.5%). Three of the 67 cases with no evidence of HER-2/neu gene amplification by FISH were moderately positive (2+) by IHC. A close relationship was observed between these 2 assays as applied to the TMA (95.4% concordance: 95% CI, - 2.2% to 6.8%; P
Original languageEnglish
Pages (from-to)362-368
Number of pages7
JournalHuman Pathology
Issue number4
Publication statusPublished - 01 Apr 2003

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


Dive into the research topics of 'Evaluation of HER-2/neu oncogene status in breast tumors on tissue microarrays'. Together they form a unique fingerprint.

Cite this