Evaluation of the electrochemical signal enhancement for domoic acid detection in shellfish using various nanomaterials

Joost Nelis, Davide Migliorelli , Safiye Jafari , Christopher Elliott, Katrina Campbell

Research output: Contribution to conferencePosterpeer-review

Abstract

Nanomaterials (NMs) are often used to functionalize and increase carbon screen printed electrode (c-SPE) performance. However, lengthy functionalisation steps such as drop-casting multiple layers or electrodeposition of NM, as well as material cost, limits interest in commercialisation of NM based SPEs. Thus, careful optimization of functionalisation protocols and cost reduction might improve future NM use for commercial biosensors.
Here, exemplary NMs were chosen to modify the c-SPE which is used as electrochemical platform for the immunosensor, based on a competitive indirect test, coupled to chronoamperometry, for the detection of domoic acid (DA). c-SPE performance was investigated for low cost solutions containing carbon black (CB), gold nanospheres (GNPs) and gold nanostars (GNSTs) (fig. 1). GNPs and GNSTs were mixed into printing ink and GNPs, GNSTs and CBs were drop-casted onto SPEs at various concentrations. The two best performing optimized electrodes (single-layer drop-casted CB-SPE and NP-SPE), and c-SPEs, were then used as platform for the immunosensor. The performance of those modified SPEs was then compared with an Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, where functionalised magnetic beads (MB) are used as platform for the immunoassay and the screen-printed electrodes as electrochemical transducers.
This study shows the merit of optimization for future commercial application of NMs in electrochemical sensing.
Original languageEnglish
Publication statusPublished - 18 Oct 2018

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