Exceptional antineoplastic activity of a dendritic-cell-targeted vaccine loaded with a Listeria peptide proposed against metastatic melanoma

Ricardo Calderon-Gonzalez, Lucia Bronchalo-Vicente, Javier Freire, Elisabet Frande-Cabanes, Lidia Alaez-Alvarez, Jose Javier Gomez-Roman, Sonsoles Yañez-Diaz, Carmen Alvarez-Dominguez

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)
3 Downloads (Pure)

Abstract

Vaccination with dendritic cells (DCs) is proposed to induce lasting responses against melanoma but its survival benefit in patients needs to be demonstrated. We propose a DC-targeted vaccine loaded with a Listeria peptide with exceptional anti-tumour activity to prevent metastasis of melanoma. Mice vaccinated with vaccines based on DCs loaded with listeriolysin O peptide (91-99) (LLO91-99) showed clear reduction of metastatic B16OVA melanoma size and adhesion, prevention of lung metastasis, enhanced survival, and reversion of immune tolerance. Robust innate and specific immune responses explained the efficiency of DC-LLO91-99 vaccines against B16OVA melanoma. The noTable features of this vaccine related to melanoma reduction were: expansion of immune-dominant LLO91-99-specific CD8 T cells that helped to expand melanoma-specific CD8+ T cells; high numbers of tumour-infiltrating lymphocytes with a cytotoxic phenotype; and a decrease in CD4+CD25high regulatory T cells. This vaccine might be a useful alternative treatment for advanced melanoma, alone or in combination with other therapies.
Original languageEnglish
Pages (from-to)16855-16865
Number of pages11
JournalOncotarget
Volume7
Issue number13
DOIs
Publication statusPublished - 01 Mar 2016
Externally publishedYes

Keywords

  • Listeria peptides
  • Dendritic cells
  • Immunotherapy
  • Melanoma
  • Vaccines

Fingerprint Dive into the research topics of 'Exceptional antineoplastic activity of a dendritic-cell-targeted vaccine loaded with a Listeria peptide proposed against metastatic melanoma'. Together they form a unique fingerprint.

Cite this