Exceptional antineoplastic activity of a dendritic-cell-targeted vaccine loaded with a Listeria peptide proposed against metastatic melanoma

Hector Teran-Navarro, Ricardo Calderon Gonzalez, Elisabet Frande-Cabanes, Carmen Alvarez-Dominguez, Sonsoles Yañez-Diaz, Javier Freire, Jose Javier Gomez-Roman

Research output: Contribution to conferencePosterpeer-review

Abstract

Vaccination with dendritic cells (DCs) is proposed to induce lasting responses against melanoma but its survival benefit in patients needs to be demonstrated. We propose a DC-targeted vaccine loaded with a Listeria peptide with exceptional anti-tumour activity to prevent metastasis of melanoma. Mice vaccinated with vaccines based on DCs loaded with listeriolysin O peptide (91--99) (LLO91–99) showed clear reduction of metastatic B16OVA melanoma size and adhesion, prevention of lung metastasis, enhanced survival, and reversion of immune tolerance. Robust innate and specific immune responses explained the efficiency of DC-LLO91–99 vaccines against B16OVA melanoma. The notable features of this vaccine related to melanoma reduction were: expansion of immune-dominant LLO91–99-specific CD8 T cells that helped to expand melanoma-specific CD8+ T cells; high numbers of tumour-infiltrating lymphocytes with a cytotoxic phenotype; and a decrease in CD4+CD25high regulatory T cells. This vaccine might be a useful alternative treatment for advanced melanoma, alone or in combination with other therapies.
Original languageEnglish
Publication statusPublished - 16 Jun 2016
Externally publishedYes
EventEMBO Conference: Problems of Listeriosis ISOPOL XIX - Institut Pasteur, Paris, France
Duration: 14 Jun 201617 Jun 2016
Conference number: XIX
https://research.pasteur.fr/en/event/isopol-xix/

Conference

ConferenceEMBO Conference: Problems of Listeriosis ISOPOL XIX
Abbreviated titleISOPOL
CountryFrance
CityParis
Period14/06/201617/06/2016
Internet address

Fingerprint Dive into the research topics of 'Exceptional antineoplastic activity of a dendritic-cell-targeted vaccine loaded with a Listeria peptide proposed against metastatic melanoma'. Together they form a unique fingerprint.

Cite this