Excess electron interactions with solvated DNA nucleotides: Strand breaks possible at room temperature

M. Smyth, J. Kohanoff

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

When biological matter is subjected to ionizing radiation, a wealth of secondary low-energy (<20 eV) electrons are produced. These electrons propagate inelastically, losing energy to the medium until they reach energies low enough to localize in regions of high electron affinity. We have recently shown that in fully solvated DNA fragments, nucleobases are particularly attractive for such excess electrons. The next question is what is their longer-term effect on DNA. It has been advocated that they can lead to strand breaks by cleavage of the phosphodiester C-3'-O-3' bond. Here we present a first-principles study of free energy barriers for the cleavage of this bond in fully solvated nucleotides. We have found that except for dAMP, the barriers are on the order of 6 kcal/mol, suggesting that bond cleavage is a regular feature at 300 K. Such low barriers are possible only as a result of solvent and thermal fluctuations. These findings support the notion that low-energy electrons can indeed lead to strand breaks in DNA.
Original languageEnglish
Pages (from-to)9122-9125
Number of pages4
JournalJournal of the American Chemical Society
Volume134
Issue number22
Early online date18 May 2012
DOIs
Publication statusPublished - 06 Jun 2012

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

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