Exogenous RNA interference exposes contrasting roles for sugar exudation in host-finding by plant pathogens

Neil D Warnock, Leonie Wilson, Juan V Canet-Perez, Thomas Fleming, Colin C Fleming, Aaron G Maule, Johnathan J Dalzell

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)
243 Downloads (Pure)

Abstract

Plant parasitic nematodes (PPN) locate host plants by following concentration gradients of root exudate chemicals in the soil. We present a simple method for RNA interference (RNAi)-induced knockdown of genes in tomato seedling roots, facilitating the study of root exudate composition, and PPN responses. Knockdown of sugar transporter genes, STP1 and STP2, in tomato seedlings triggered corresponding reductions of glucose and fructose, but not xylose, in collected root exudate. This corresponded directly with reduced infectivity and stylet thrusting of the promiscuous PPN Meloidogyne incognita, however we observed no impact on the infectivity or stylet thrusting of the selective Solanaceae PPN Globodera pallida. This approach can underpin future efforts to understand the early stages of plant-pathogen interactions in tomato and potentially other crop plants.

Original languageEnglish
Number of pages5
JournalInternational Journal for Parasitology
Early online date29 Mar 2016
DOIs
Publication statusEarly online date - 29 Mar 2016

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