Experimental and computational examination of anastellin (FnIII1c)–polymer interactions

David Mallinson, David L. Cheung*, Dorin Simionesie, Alexander B. Mullen, Zhenyu J. Zhang, Dimitrios A. Lamprou

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)
17 Downloads (Pure)

Abstract

Using a combination of experimental and computational approaches, the interaction between anastellin, a recombinant fragment of fibronectin, and representative biomaterial surfaces has been examined. Anastellin and superfibronectin have been seen to exhibit antiangiogenic properties and other properties that may make it suitable for consideration for incorporation into biomaterials. The molecular interaction was directly quantified by atomic force microscope (AFM)-based force spectroscopy, complemented by adsorption measurements using quartz crystal microbalance (QCM). Using AFM, it was found that the anastellin molecule facilitates a stronger adhesion on polyurethane films (72.0 pN nm−1) than on poly (methyl methacrylate) films (68.6 pN nm−1). However, this is not consistent with the QCM adsorption measurements, which show no significant difference. Molecular dynamics simulations of the behavior of anastellin on polyurethane in aqueous solution were performed to rationalize the experimental data, and show that anastellin is capable of rapid adsorption to PU while its secondary structure is stable upon adsorption in water.

Original languageEnglish
Pages (from-to)737-745
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume105
Issue number3
Early online date07 Nov 2016
DOIs
Publication statusPublished - Mar 2017
Externally publishedYes

Keywords

  • atomic force microscopy
  • fibronectin
  • molecular dynamics
  • poly (methyl methacrylate)
  • polyurethane

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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