Explore protein molecular structure in endosperm tissues in newly developed black and yellow type canola seeds by using synchrotron-based Fourier transform infrared microspectroscopy

Katerina Theodoridou, Sally Vail, Peiqiang Yu*

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This study was conducted to characterize the protein molecular structure in endosperm tissues in newly developed black and yellow-type canola seeds by using synchrotron-based Fourier transform infrared microspectroscopy. The results showed that the yellow canola seeds contained relatively lower (P < 0.05) percentage of β-sheet and amide I and amide II area compared to the black-type canola seed. This might be an indication that the protein value of the yellow canola seeds as food or feed is different from that of the black canola seeds. The multivariate molecular spectral analyses (AHCA, PCA) showed that there were not significant molecular structural differences in the protein amide I and amide II fingerprint region (ca. 1720-1480 cm-1) between the yellow and the black-type of canola seed. It can be concluded that both the yellow and the black-seeded canola contain the same proteins but in different ratios.

Original languageEnglish
Pages (from-to)421-427
Number of pages7
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume120
Early online date22 Oct 2013
DOIs
Publication statusPublished - 24 Feb 2014
Externally publishedYes

Keywords

  • Amide I
  • Canola seed
  • Protein structure
  • Synchrotron IR microspectroscopy
  • α-Helix
  • β-Sheet

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy

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