Expression of complement components and regulators by different subtypes of bone marrow-derived macrophages

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21 Citations (Scopus)

Abstract

Under inflammatory conditions, macrophages can differentiate into different functional subtypes. We show that bone marrow-derived macrophages constitutively express different levels of various complement-related genes. The relative expression levels are C1qb > Crry > CFH > C3 > C1r > CFB > DAF1 > CD59a > C2 > C1INH > C1s > C4. Upon activation, the expression of C1r, C1s, C3, C2, CFB, and C1INH was up-regulated, and CFH, CD59a, and DAF1, down-regulated in M1 (induced by interferon-? + lipopolysaccharides (LPS)) and M2b (induced by immune complex + LPS) macrophages. The expression of C4 and CFH was slightly up-regulated in interleukin (IL)-10-induced M2c macrophages. Complement gene expression in IL-4-induced M2a macrophages was weakly down-regulated as compared to resting M0 macrophages. Higher levels of C3, C1INH, and CFB but lower levels of CFH expression in M1 and M2b macrophage suggests that they may be involved in the alternative pathway of complement activation during inflammation.
Original languageEnglish
Pages (from-to)1448-1461
Number of pages14
JournalInflammation
Volume35
Issue number4
Early online date27 Mar 2012
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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