Expression of the VEGF gene family during retinal vaso-obliteration and hypoxia.

David Simpson, Grainne M. Murphy, T. Bhaduri, Tom Gardiner, Desmond Archer, Alan Stitt

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Vascular insufficiency and retinal ischaemia precede many proliferative retinopathies and stimulate secretion of vasoactive growth factors. Vascular endothelial growth factor (VEGF) plays a major role and we therefore investigated the other members of the VEGF family: Placental growth factor (PlGF), VEGF-B, -C, and -D, and platelet derived growth factors (PDGF) A and B. Neonatal mice were exposed to hyperoxia for 5 days and then returned to room air (resulting in acute retinal ischaemia). RT-PCR demonstrated that all the members of the VEGF family are expressed in the retina and in situ hybridization (ISH) located their mRNAs primarily in ganglion cells. Similarly to VEGF itself, VEGF-C, PDGF-A, and PDGF-B were upregulated during retinal ischaemia (P < 0.05). Only PlGF gene expression increased during hyperoxia (P < 0.01). The expression pattern of these growth factors suggests a role in the normal retina and during vaso-obliterative and ischaemic phases.
Original languageEnglish
Pages (from-to)333-340
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume262
Issue number2
DOIs
Publication statusPublished - 27 Aug 1999

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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